CoraLite®555-conjugated CDC6 Monoclonal antibody

CDC6 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

6F12D2

Cat no : CL555-66021

Synonyms

Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6)

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Validation Data Gallery

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  FC experiment of HepG2 using CL555-66021

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CDC6 (CL555-66021, Clone:6F12D2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL555-66021 is CDC6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDC6 fusion protein Ag17958
• Full Name: cell division cycle 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 560 aa, 63 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC025232
• Gene Symbol: CDC6
• Gene ID (NCBI): 990
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.