CFTR Polyclonal antibody, PBS Only

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• CFTR Polyclonal antibody, PBS Only (20738-1-PBS)

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  IHC staining of human lung using 20738-1-AP (same clone as 20738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20738-1-AP (CFTR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20738-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 20738-1-AP (same clone as 20738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20738-1-AP (CFTR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20738-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20738-1-AP (same clone as 20738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20738-1-AP (CFTR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20738-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 20738-1-AP (same clone as 20738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20738-1-AP (CFTR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20738-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 20738-1-AP (same clone as 20738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20738-1-AP (CFTR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20738-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 20738-1-AP (same clone as 20738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20738-1-AP (CFTR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20738-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 20738-1-AP (same clone as 20738-1-PBS)

  1X10^6 HUVEC cells were intracellularly stained with 0.2 ug Anti-Human CFTR (20738-1-AP) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20738-1-PBS in a different storage buffer formulation.

CFTR Polyclonal Antibody for IHC, FC (Intra), Indirect ELISA

Cat No. 20738-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra), Indirect ELISA

Synonyms

ABCC7, ATP-binding cassette sub-family C member 7, cAMP-dependent chloride channel, CFTR/MRP, Channel conductance-controlling ATPase

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Product Information

20738-1-PBS targets CFTR in IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
• Calculated Molecular Weight: 168 kDa
• GenBank Accession Number: NM_000492
• Gene Symbol: CFTR
• Gene ID (NCBI): 1080
• RRID: AB_2722558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13569
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

      What is the human cytogenetic location of the CFTR gene?

7q31.2

What is the predicted molecular weight of CFTR? 

168 kDa

Where is CFTR expressed?

CFTR is mainly expressed in apical membranes of vertebrate epithelial cells.

What is the ion selectivity of CFTR?

CFTR is an anion-selective channel, responsible for regulating chloride and bicarbonate transport.

What is the process of CFTR trafficking?

The ER provides the first steps in CFTR folding quality control. CFTR can be degraded by the ER associated degradation (ERAD) pathway, or it is transported to the Golgi for maturation before being trafficked to the cell membrane.

What are the separate domains of CFTR?

CFTR consists of two transmembrane domains, two nucleotide binding domains, and a regulatory domain.

What regulates CFTR?

CFTR activity is ATP-dependent and is regulated by protein kinase A phosphorylation.

What is the role of CFTR?

CFTR functions as a channel for anions, regulating water and ionic homeostasis.

What mutations in CFTR leads to protein degradation?

Deletion of the regulatory domain or the phenylalanine 508 (F508del) results in an increase in CFTR degradation. This degradation then leads to a decrease of secretion product clearance and an increase in viscous mucosal buildup at the epithelial surface of affected organs, the primary symptom of cystic fibrosis. Mutations in CFTR can also lead congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities in men.

How does CFTR differ from other ATP-binding cassette proteins?

Unlike other proteins in the ABC family, CFTR functions as an ion channel.

PMIDs: 24513531, 24534272, 22966013, 22709980