CoraLite®488-conjugated CHST4 Monoclonal antibody

CHST4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1E1A4

Cat no : CL488-66623

Synonyms

CHST4, GlcNAc6ST 2, Gn6st 2, GST 3, HEC GlcNAc6ST, LSST



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66623-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66623 targets CHST4 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHST4 fusion protein Ag3987
Full Name carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
Calculated molecular weight 370 aa, 43 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC035282
Gene symbol CHST4
Gene ID (NCBI) 10164
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)

Protocols

Product Specific Protocols
IF protocol for CL488 CHST4 antibody CL488-66623Download protocol
Standard Protocols
Click here to view our Standard Protocols