CHST4 Monoclonal antibody

CHST4 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1E1A4

Cat no : 66623-1-Ig

Synonyms

CHST4, GlcNAc6ST 2, Gn6st 2, GST 3, HEC GlcNAc6ST, LSST



Tested Applications

Positive WB detected inHT-29 cells, T-47D cells, MCF-7 cells, COLO 320 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66623-1-Ig targets CHST4 in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHST4 fusion protein Ag3987
Full Name carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
Calculated molecular weight 370 aa, 43 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC035282
Gene symbol CHST4
Gene ID (NCBI) 10164
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)

Protocols

Product Specific Protocols
WB protocol for CHST4 antibody 66623-1-IgDownload protocol
IHC protocol for CHST4 antibody 66623-1-IgDownload protocol
IF protocol for CHST4 antibody 66623-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IHC

Front Oncol

Carbohydrate Sulfotransferase 4 Inhibits the Progression of Hepatitis B Virus-Related Hepatocellular Carcinoma and Is a Potential Prognostic Marker in Several Tumors.

Authors - Longshan Zhang
  • KD Validated
humanIHC

Genomics Proteomics Bioinformatics

miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma.

Authors - Bo Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

DAN (Verified Customer) (08-19-2019)

the CHST4 antibody doesn't work as expected

  • Applications: Western Blot,