CHST4 Monoclonal antibody

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  WB analysis of HT-29 using 66623-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 66623-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66623-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66623-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66623-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66623-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66623-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CHST4 antibody (66623-1-Ig, Clone: 1E1A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HeLa using 66623-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66623-1-Ig (CHST4 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

CHST4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66623-1-Ig

Clone No.1E1A4

Publications(3)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Gn6st-2, Gn6st 2, GlcNAc6ST-2, GlcNAc6ST 2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3

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Tested Applications

• Positive WB detected in: HT-29 cells, T-47D cells, MCF-7 cells, COLO 320 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

66623-1-Ig targets CHST4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3987

  Product name: Recombinant human CHST4 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 177-386 aa of BC035282

  Sequence: DPSLNLHIVHLVRDPRAVFRSRERTKGDLMIDSRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH

• Full Name: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
• Calculated Molecular Weight: 370 aa, 43 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC035282
• Gene Symbol: CHST4
• Gene ID (NCBI): 10164
• RRID: AB_2881983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8NCG5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Genomics Proteomics Bioinformatics; miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma.; Authors - Bo Hu
mouse,human	WB,IHC	Front Oncol; Carbohydrate Sulfotransferase 4 Inhibits the Progression of Hepatitis B Virus-Related Hepatocellular Carcinoma and Is a Potential Prognostic Marker in Several Tumors.; Authors - Longshan Zhang ; KD Validated
human	IHC	J Leukoc Biol; CHST4 associates with high-abundance immune infiltration in hormone receptor-positive breast cancer; Authors - Yansong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

DAN (Verified Customer) (08-19-2019)

the CHST4 antibody doesn't work as expected

• Applications: Western Blot,