|Positive WB detected in||HT-29 cells, T-47D cells, MCF-7 cells, COLO 320 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66623-1-Ig targets CHST4 in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CHST4 fusion protein Ag3987|
|Full Name||carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4|
|Calculated molecular weight||370 aa, 43 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC035282|
|Gene ID (NCBI)||10164|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)
Carbohydrate Sulfotransferase 4 Inhibits the Progression of Hepatitis B Virus-Related Hepatocellular Carcinoma and Is a Potential Prognostic Marker in Several Tumors.
Genomics Proteomics Bioinformatics
miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
DAN (Verified Customer) (08-19-2019)
the CHST4 antibody doesn't work as expected