• Featured Product
  • KD/KO Validated

CHST4 Monoclonal antibody

CHST4 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human and More (2)







Cat no : 66623-1-Ig


CHST4, GlcNAc6ST 2, Gn6st 2, GST 3, HEC GlcNAc6ST, LSST

Tested Applications

Positive WB detected inHT-29 cells, T-47D cells, MCF-7 cells, COLO 320 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66623-1-Ig targets CHST4 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHST4 fusion protein Ag3987
Full Name carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
Calculated Molecular Weight 370 aa, 43 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC035282
Gene Symbol CHST4
Gene ID (NCBI) 10164
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)


Product Specific Protocols
WB protocol for CHST4 antibody 66623-1-IgDownload protocol
IHC protocol for CHST4 antibody 66623-1-IgDownload protocol
IF protocol for CHST4 antibody 66623-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Genomics Proteomics Bioinformatics

miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma.

Authors - Bo Hu

Front Oncol

Carbohydrate Sulfotransferase 4 Inhibits the Progression of Hepatitis B Virus-Related Hepatocellular Carcinoma and Is a Potential Prognostic Marker in Several Tumors.

Authors - Longshan Zhang
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


DAN (Verified Customer) (08-19-2019)

the CHST4 antibody doesn't work as expected

  • Applications: Western Blot,