Collagen Type VII Polyclonal antibody

Collagen Type VII Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19799-1-AP

Synonyms

COL7A1, Collagen alpha 1(VII) chain, Collagen Type VII, collagen, type VII, alpha 1, EBD1, EBDCT, EBR1, LC collagen, Long chain collagen

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Validation Data Gallery

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  WB analysis using 19799-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19799-1-AP (Collagen Type VII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19799-1-AP targets Collagen Type VII in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: collagen, type VII, alpha 1
• Calculated Molecular Weight: 295 kDa
• Observed Molecular Weight: 290 kDa
• GenBank Accession Number: NM_000094
• Gene Symbol: COL7A1
• Gene ID (NCBI): 1294
• RRID: AB_2878609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL7A1, also named as LC collagen, is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica (DEB). Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica Pasini type (P-DEB) which also known as albopapuloid dominant dystrophic epidermolysis bullosa. Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica Hallopeau-Siemens type (HS-DEB). Defects in COL7A1 are the cause of transient bullous dermolysis of the newborn (TBDN). Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica pretibial type (PR-DEB). Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica Bart type (B-DEB). Defects in COL7A1 are the cause of epidermolysis bullosa pruriginosa (EBP). Defects in COL7A1 are the cause of isolated toenail dystrophy without skin fragility. Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica with subcorneal cleavage (EBDSC) which also known as epidermolysis bullosa simplex superficialis (EBSS). This antibody is specific to COL7A1.