CPLX1 Recombinant antibody

CPLX1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85874-1-RR
Clone No.250131A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Complexin-1, CPLX 1, CPX I, CPX1, CPXI

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85874-1-RR targets CPLX1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CPLX1 fusion protein Ag0387 Predict reactive species
Full Name complexin 1
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15-20 kDa
GenBank Accession NumberBC002471
Gene Symbol CPLX1
Gene ID (NCBI) 10815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14810
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complexin 1 (CPLX1) is a member of the complexin/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders.

Protocols

Product Specific Protocols
WB protocol for CPLX1 antibody 85874-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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