CPLX1 Recombinant antibody

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  WB analysis of SH-SY5Y using 85874-1-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85874-1-RR (CPLX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85874-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85874-1-RR (CPLX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

CPLX1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85874-1-RR

Clone No.250131A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Complexin-1, CPLX 1, CPX I, CPX1, CPXI

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85874-1-RR targets CPLX1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CPLX1 fusion protein Ag0387
• Full Name: complexin 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC002471
• Gene Symbol: CPLX1
• Gene ID (NCBI): 10815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14810
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complexin 1 (CPLX1) is a member of the complexin/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders.