CoraLite® Plus 488-conjugated Calponin Polyclonal antibody
Calponin Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-13938
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | C2C12 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-13938 targets Calponin in IF applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Calponin fusion protein Ag4989 |
Full Name | calponin 1, basic, smooth muscle |
Calculated Molecular Weight | 33 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC022015 |
Gene Symbol | CNN1 |
Gene ID (NCBI) | 1264 |
RRID | AB_2919081 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Calponin antibody CL488-13938 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |