Tested Applications
| Positive WB detected in | mouse colon tissue, mouse heart tissue, mouse lung tissue, mouse uterus tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | C2C12 cells, H9C2 cells |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13938-1-AP targets Calponin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4989 Product name: Recombinant human Calponin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC022015 Sequence: MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNFMDGLKDGIILCEFINKLQPGSVKKINESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNVGVKYAEKQERKFEPGKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPRQVYDPKYCLTPEYPELGEPAHNHHAHNYYNSA Predict reactive species |
| Full Name | calponin 1, basic, smooth muscle |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC022015 |
| Gene Symbol | Calponin |
| Gene ID (NCBI) | 1264 |
| RRID | AB_2082010 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51911 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Calponin antibody 13938-1-AP | Download protocol |
| IF protocol for Calponin antibody 13938-1-AP | Download protocol |
| IHC protocol for Calponin antibody 13938-1-AP | Download protocol |
| IP protocol for Calponin antibody 13938-1-AP | Download protocol |
| WB protocol for Calponin antibody 13938-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Calponin (CNN1) polyclonal antibody (Cat# 13938-1-AP) has 77 citations published in journals including Nature Communications, Journal of Clinical Investigation, Advanced Materials, Biomaterials, Atherosclerosis, and Journal of the American Heart Association. Research fields predominantly involve vascular smooth muscle cell phenotypic switching, atherosclerosis, aortic aneurysm and dissection, vascular remodeling, erectile dysfunction, airway smooth muscle pathology, and tissue-engineered vascular grafts.
| Species | Application | Title |
|---|---|---|
Adv Mater Safeguarding VSMC Contractile Phenotype With In Situ circRNA-mediated Endothelial Olaratumab Engineering to Prevent Vascular Graft Stenosis. | ||
Nat Commun Improving erectile function in diabetic male mice by rescuing depalmitoylated FBP1 to reduce cavernosal lactate. | ||
Nat Commun SLC1A5 prevents aortic aneurysm and dissection by glutaminolytic-epigenetic orchestration of vascular smooth muscle cell homeostasis. | ||
Circ Res Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System. | ||
J Adv Res Cytoskeletal-related genes function as checkpoints for the maintenance of VSMC contractile phenotype and prevent pathological remodeling in arterial diseases | ||
Redox Biol Vascular smooth muscle-MAPK14 is required for neointimal hyperplasia by suppressing VSMC differentiation and inducing proliferation and inflammation. |
Reviews
What customers say
Both reviewers used this antibody for Western Blot and reported positive results. One user (5/5) tested it at 1:1000 dilution on HPASMC cells and noted it works well and can be reused when stored at 4°C. The other (4/5) validated it on mouse primary cardiomyocytes, describing it as a "very good antibody." Overall, users find it reliable for WB applications across different cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yajing (Verified Customer) (11-12-2019) | The antibody works well. Can be reused when stored at 4 degrees.
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Ying (Verified Customer) (08-19-2019) | very good antibody
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