|Positive WB detected in||mouse heart tissue, mouse lung tissue|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||human heart tissue, human bladder tissue, human breast cancer tissue, human hysteromyoma tissue, human ovary tumor tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13938-1-AP targets Calponin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calponin fusion protein Ag4989|
|Full Name||calponin 1, basic, smooth muscle|
|Calculated molecular weight||33 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC022015|
|Gene ID (NCBI)||1264|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.
|Product Specific Protocols|
|WB protocol for Calponin antibody 13938-1-AP||Download protocol|
|IHC protocol for Calponin antibody 13938-1-AP||Download protocol|
|IF protocol for Calponin antibody 13938-1-AP||Download protocol|
|IP protocol for Calponin antibody 13938-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yajing (Verified Customer) (11-12-2019)
The antibody works well. Can be reused when stored at 4 degrees.
Ying (Verified Customer) (08-19-2019)
very good antibody