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calreticulin Polyclonal antibody
calreticulin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 27298-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, C6 cells, mouse skeletal muscle tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells |
Positive IHC detected in | human thyroid tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, SKOV-3 cells, HeLa cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 13 publications below |
IHC | See 1 publications below |
IF | See 21 publications below |
FC | See 2 publications below |
Product Information
27298-1-AP targets calreticulin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | calreticulin fusion protein Ag26064 |
Full Name | calreticulin |
Calculated molecular weight | 60 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC002500 |
Gene symbol | CALR |
Gene ID (NCBI) | 811 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.
Protocols
Product Specific Protocols | |
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WB protocol for calreticulin antibody 27298-1-AP | Download protocol |
IHC protocol for calreticulin antibody 27298-1-AP | Download protocol |
IF protocol for calreticulin antibody 27298-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK. | ||
Nat Commun In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses | ||
Nat Commun Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer. | ||
ACS Appl Mater Interfaces Cascade-Responsive Hierarchical Nanosystems for Multisite Specific Drug Exposure and Boosted Chemoimmunotherapy | ||
ACS Appl Mater Interfaces A Versatile Nanoplatform for Broad-Spectrum Immunotherapy by Reversing the Tumor Microenvironment | ||
ACS Appl Mater Interfaces Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform |