calreticulin Polyclonal antibody

• Featured Product
• KD/KO Validated

calreticulin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

57

Cat no : 27298-1-AP

Synonyms

CALR, Calregulin

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Validation Data Gallery

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  WB analysis of HeLa using 27298-1-AP

  WB result of Calreticulin antibody (27298-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calreticulin transfected HeLa cells.

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  WB analysis using 27298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27298-1-AP (Calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27298-1-AP (Calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 27298-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 27298-1-AP (calreticulin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 27298-1-AP (calreticulin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 27298-1-AP (calreticulin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human normal colon using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27298-1-AP (calreticulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27298-1-AP (calreticulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calreticulin antibody (27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calreticulin antibody (27298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

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  IF Staining of HepG2 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using calreticulin antibody (27298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of SKOV-3 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Calreticulin antibody (27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 27298-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug calreticulin Polyclonal antibody (27298-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, C6 cells, mouse skeletal muscle tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells
• Positive IHC detected in: human thyroid tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, SKOV-3 cells, HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 39 publications below
• FC: See 2 publications below

Product Information

27298-1-AP targets calreticulin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: calreticulin fusion protein Ag26064
• Full Name: calreticulin
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC002500
• Gene Symbol: Calreticulin
• Gene ID (NCBI): 811
• RRID: AB_2880835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Publications

Species	Application	Title
human	WB	Cell Metab; Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK.; Authors - Mengqi Li
mouse	IF	Nat Commun; In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses; Authors - Yuxin Guo
mouse	FC,IF	Nat Commun; Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.; Authors - Yanjuan Huang
human	WB	EMBO J; STING directly recruits WIPI2 for autophagosome formation during STING-induced autophagy; Authors - Wei Wan
mouse		J Control Release; Positron emission tomography guided synergistic treatment of melanoma using multifunctional zirconium-hematoporphyrin nanosonosensitizers; Authors - Xiaodan Jiao
human,mouse	IF,WB	ACS Appl Mater Interfaces; A Versatile Nanoplatform for Broad-Spectrum Immunotherapy by Reversing the Tumor Microenvironment; Authors - Ao Chen