|Positive WB detected in||C6 cells, HeLa cells, mouse skeletal muscle tissue, NIH/3T3 cells|
|Positive IHC detected in||human thyroid tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells, SKOV-3 cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 27298-1-AP is Calreticulin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calreticulin fusion protein Ag26064|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC002500|
|Gene ID (NCBI)||811|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.
|Product Specific Protocols|
|WB protocol for Calreticulin antibody 27298-1-AP||Download protocol|
|IHC protocol for Calreticulin antibody 27298-1-AP||Download protocol|
|IF protocol for Calreticulin antibody 27298-1-AP||Download protocol|
|FC protocol for Calreticulin antibody 27298-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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