calreticulin Polyclonal antibody

• Featured Product
• KD/KO Validated

calreticulin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 27298-1-AP

Synonyms

CALR, Calregulin, calreticulin, cC1qR, CRP55, CRT, CRTC, ERp60, grp60, HACBP, RO, SSA

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Validation Data Gallery

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  WB analysis of HeLa using 27298-1-AP

  WB result of Calreticulin antibody (27298-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calreticulin transfected HeLa cells.

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  WB analysis using 27298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27298-1-AP (Calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27298-1-AP (Calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 27298-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 27298-1-AP (calreticulin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 27298-1-AP (calreticulin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 27298-1-AP (calreticulin Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calreticulin antibody (27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calreticulin antibody (27298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

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  IF Staining of SKOV-3 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Calreticulin antibody (27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 27298-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Calreticulin antibody (27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 27298-1-AP

  1X10^6 Jurkat cells were stained with 0.2 ug Anti-Human Calreticulin (27298-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HeLa cells, C6 cells, mouse skeletal muscle tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells
• Positive IHC detected in: human thyroid tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, HeLa cells, SKOV-3 cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 17 publications below
• FC: See 2 publications below

Product Information

The immunogen of 27298-1-AP is calreticulin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: calreticulin fusion protein Ag26064
• Full Name: calreticulin
• Calculated molecular weight: 60 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC002500
• Gene symbol: CALR
• Gene ID (NCBI): 811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Publications

Species	Application	Title
human	WB	Cell Metab; Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK.; Authors - Mengqi Li
mouse	FC,IF	Nat Commun; Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.; Authors - Yanjuan Huang
mouse	IF	Theranostics; Ultrasound (US)-activated redox dyshomeostasis therapy reinforced by immunogenic cell death (ICD) through a mitochondrial targeting liposomal nanosystem; Authors - Junjie Ren
mouse	IF	J Nanobiotechnology; Peptide vaccine-conjugated mesoporous carriers synergize with immunogenic cell death and PD-L1 blockade for amplified immunotherapy of metastatic spinal.; Authors - Zhenqing Wang
human	WB,IF	J Nanobiotechnology; A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases.; Authors - Na Qiu
human	IF	Biomater Sci; Tumor-targeting oxidative stress nanoamplifiers as anticancer nanomedicine with immunostimulating activity; Authors - Nanhee Song