Cytochrome c Recombinant antibody

Cytochrome c Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG


Human, Mouse







Cat no : 83276-1-RR


CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, mouse kidney tissue
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83276-1-RR is Cytochrome c Fusion Protein expressed in E. coli.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cytochrome c fusion protein Ag1455
Full Name cytochrome c, somatic
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12-15 kDa
GenBank Accession NumberBC009578
Gene Symbol CYCS
Gene ID (NCBI) 54205
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.


Product Specific Protocols
WB protocol for Cytochrome c antibody 83276-1-RRDownload protocol
FC protocol for Cytochrome c antibody 83276-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols