CoraLite® Plus 488-conjugated DAPK1 Monoclonal antibody

DAPK1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2F9

Cat no : CL488-67815

Synonyms

DAP kinase 1, DAPK, DAPK1, DKFZp781I035

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Validation Data Gallery

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  IF Staining of human breast cancer using CL488-67815

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 DAPK1 antibody (CL488-67815, Clone: 1E2F9 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL488-67815

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 DAPK1 antibody (CL488-67815, Clone: 1E2F9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67815 targets DAPK1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DAPK1 fusion protein Ag29838
• Full Name: death-associated protein kinase 1
• Calculated Molecular Weight: 1430 aa, 160 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC113660
• Gene Symbol: DAPK1
• Gene ID (NCBI): 1612
• RRID: AB_2919555
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784).