DAPK1 Monoclonal antibody

DAPK1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1E2F9

Cat no : 67815-1-Ig

Synonyms

DAP kinase 1, DAPK, DAPK1, DKFZp781I035



Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67815-1-Ig targets DAPK1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DAPK1 fusion protein Ag29838
Full Name death-associated protein kinase 1
Calculated molecular weight 1430 aa, 160 kDa
Observed molecular weight160 kDa
GenBank accession numberBC113660
Gene symbol DAPK1
Gene ID (NCBI) 1612
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784).

Protocols

Product Specific Protocols
WB protocol for DAPK1 antibody 67815-1-IgDownload protocol
IHC protocol for DAPK1 antibody 67815-1-IgDownload protocol
IF protocol for DAPK1 antibody 67815-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols