E2F1 Polyclonal antibody

• Featured Product
• KD/KO Validated

E2F1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

46

Cat no : 12171-1-AP

Synonyms

E2F 1, E2F transcription factor 1, E2F1, PBR3, PRB binding protein E2F 1, RBAP 1, RBAP1, RBBP 3, RBBP3, Transcription factor E2F1

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Validation Data Gallery

• 

  WB analysis of HeLa using 12171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12171-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12171-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12171-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12171-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12171-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12171-1-AP (E2F1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, mouse brain tissue, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 40 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

12171-1-AP targets E2F1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, Artemia sinica
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E2F1 fusion protein Ag2813
• Full Name: E2F transcription factor 1
• Calculated molecular weight: 437 aa, 47 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC050369
• Gene symbol: E2F1
• Gene ID (NCBI): 1869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 protein. The sumoylated E2F1 is bout 55 kDa.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.; Authors - Yudi Xiong
human	WB	J Cell Mol Med; Down-regulation of TFAM increases the sensitivity of tumour cells to radiation via p53/TIGAR signalling pathway.; Authors - Xu Jiang
human	WB	Oncotarget; Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1).; Authors - Zhanping Lu
human	WB	Oncotarget; Dihydroartemisinin suppresses pancreatic cancer cells via a microRNA-mRNA regulatory network.; Authors - Yilong Li
human	WB	Oncotarget; RACK1 promotes lung cancer cell growth via an MCM7/RACK1/ Akt signaling complex.; Authors - Liangru Fei
human	WB	Tumour Biol; CIP2A is associated with multidrug resistance in cervical adenocarcinoma by a P-glycoprotein pathway.; Authors - Juan Liu

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