ERCC6 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of HeLa using 86144-1-RR (same clone as 86144-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 86144-1-RR (ERCC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86144-1-PBS in a different storage buffer formulation.

ERCC6 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86144-1-PBS

Clone No.250645D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ARMD5, ATP dependent helicase ERCC6, Chimeric CSB-PGBD3 protein, Chimeric ERCC6-PGBD3 protein, CKN2

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Product Information

86144-1-PBS targets ERCC6 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18009

  Product name: Recombinant human ERCC6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1144-1493 aa of BC127104

  Sequence: DESIDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSVAEEETLEKHLRPKQKPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSVGVHSVMKHDAIMDGASPDYVLVEAEANRVAQDAPKALRLSRQRCLGAVSGVPTWTGHRGISGAPAGKKSRFGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDADSSSGPLASSSLLAKMRARNHLILPERLESESGHLQEASALLPTTEHDDLLVEMRNFIAFQAHTDGQASTREILQEFESKLSASQSCVFRELLRNLCTFHRTSGGEGIWKLKPEYC

• Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
• Calculated Molecular Weight: 1493 aa, 168 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC127104
• Gene Symbol: ERCC6/CSB
• Gene ID (NCBI): 2074
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q03468
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ERCC6, also named as CSB, belongs to the SNF2/RAD54 helicase family. It is involved in the preferential repair of active genes. It is presumed DNA or RNA unwinding function. ERCC6 corrects the UV survival and RNA synthesis after UV exposure of Cockayne syndrome complementation group B. Defects in ERCC6 are the cause of Cockayne syndrome type B (CSB) , cerebro-oculo-facio-skeletal syndrome type 1 (COFS1), De Sanctis-Cacchione syndrome (DSC), and UV-sensitive syndrome (UVS). Genetic variation in ERCC6 is associated with susceptibility to age-related macular degeneration type 5 (ARMD5). The antibody is specific to ERCC6.