FANCA Recombinant antibody

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  WB analysis using 84923-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84923-5-RR (FANCA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84923-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84923-5-RR against Human FANCA were performed. The affinity constant is 0.351 nM.

FANCA Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84923-5-RR

Clone No.242600E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FAH, FACA, FAA, FA1, FA

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, A549 cells, HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84923-5-RR targets FANCA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FANCA fusion protein Ag2598
• Full Name: Fanconi anemia, complementation group A
• Calculated Molecular Weight: 163 kDa
• Observed Molecular Weight: 163 kDa
• GenBank Accession Number: BC008979
• Gene Symbol: FANCA
• Gene ID (NCBI): 2175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15360
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FANCA, also named as FAA, FACA and FANCH, is DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. FANCA may be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Defects in FANCA are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody is specific to isoform1 of FANCA.