FCER1G Recombinant antibody

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  WB analysis using 85390-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85390-2-RR (FCER1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85390-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85390-2-RR against Human FCER1G were performed. The affinity constant is 1.16 nM.

FCER1G Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85390-2-RR

Clone No.242829E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, ELISA

Synonyms

242829E3, Fc epsilon RI gamma, Fc receptor gamma-chain, Fc-epsilon RI-gamma, FceRI gamma

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Tested Applications

• Positive WB detected in: THP-1 cells, HL-60 cells, RAW 264.7 cells, pig spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85390-2-RR targets FCER1G in WB, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FCER1G fusion protein Ag4442
• Full Name: Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
• Calculated Molecular Weight: 87 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC033872
• Gene Symbol: FcRγ
• Gene ID (NCBI): 2207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30273
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FCER1G (Fc receptor gamma-chain, FcRgamma), also known as Fc-epsilon RI-gamma, or IgE Fc receptor subunit gamma (FceRI gamma), is a component of the high-affinity immunoglobulin E (IgE) receptor (Fc epsilon RI) which is a tetrameric complex of one alpha subunit, one beta subunit, and two disulfide-linked gamma subunits (PMID: 2146219; 2531187). Fc epsilon RI is present exclusively on mast cells and basophils, mediating allergic inflammatory signaling (PMID: 17438574). FCER1G is widely expressed and contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors (PMID: 19098920).