FEZ2 Recombinant monoclonal antibody

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  FC experiment of A431 using 83407-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Fez2 Recombinant Antibody (83407-6-RR, Clone:240248F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 83407-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83407-6-RR against Human FEZ2 were performed. The affinity constant is 6.64 nM.

FEZ2 Uni-rAb^® Recombinant Antibody for FC (Intra), ELISA

Cat No. 83407-6-RR

Clone No.240248F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2

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Tested Applications

• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83407-6-RR targets FEZ2 in FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15370

  Product name: Recombinant human FEZ2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-160 aa of BC110386

  Sequence: MAADGDWQDFYEFQEPARSLLDQENCNASPEPGAEAGAGAGGGADGFPALACSLEEKLSLCFRPSDPGAEPPRTAVRPITERSLLQGDEIWNALTDNYGNVMPVDWKSSHTRTLHLLTLNLSEKGVSDSLLFDTSDDEELREQLDMHSIIVSCVNDEPLF

• Full Name: fasciculation and elongation protein zeta 2 (zygin II)
• Calculated Molecular Weight: 353 aa, 40 kDa
• GenBank Accession Number: BC110386
• Gene Symbol: FEZ2
• Gene ID (NCBI): 9637
• RRID: AB_3671054
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UHY8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles.