FTL Recombinant monoclonal antibody, PBS Only

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  WB analysis using 81166-3-RR (same clone as 81166-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81166-3-RR (FTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81166-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 81166-3-RR (same clone as 81166-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81166-3-RR (FTL antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81166-3-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 81166-3-RR (same clone as 81166-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using FTL antibody (81166-3-RR, Clone: 251048G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81166-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81166-3-RR (same clone as 81166-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FTL antibody (81166-3-RR, Clone: 251048G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81166-3-PBS in a different storage buffer formulation.

FTL Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 81166-3-PBS

Clone No.251048G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Ferritin, Ferritin light chain, Ferritin L subunit

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Product Information

81166-3-PBS targets FTL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag20637

  Product name: Recombinant human FTL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-175 aa of BC008439

  Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD

• Full Name: ferritin, light polypeptide
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC008439
• Gene Symbol: Ferritin light chain
• Gene ID (NCBI): 2512
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02792
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.