FXYD1 Monoclonal antibody

FXYD1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C10G9

Cat no : 67150-1-Ig

Synonyms

FXYD1, Phospholemman, PLM

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Validation Data Gallery

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  WB analysis of human heart using 67150-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 67150-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67150-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 67150-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67150-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 67150-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 67150-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human heart tissue, human skeletal muscle tissue, pig heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67150-1-Ig targets FXYD1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, mouse samples.

• Tested Reactivity: Human, Pig, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FXYD1 fusion protein Ag25307
• Full Name: FXYD domain containing ion transport regulator 1
• Calculated Molecular Weight: 92 aa, 10 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC032800
• Gene Symbol: FXYD1
• Gene ID (NCBI): 5348
• RRID: AB_2882448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)