FXYD1 Monoclonal antibody

FXYD1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C10G9

Cat no : 67150-1-Ig

Synonyms

FXYD1, Phospholemman, PLM



Tested Applications

Positive WB detected inhuman heart tissue, human skeletal muscle tissue, pig heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67150-1-Ig targets FXYD1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, mouse samples.

Tested Reactivity Human, Pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FXYD1 fusion protein Ag25307
Full Name FXYD domain containing ion transport regulator 1
Calculated molecular weight 92 aa, 10 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC032800
Gene symbol FXYD1
Gene ID (NCBI) 5348
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

Protocols

Product Specific Protocols
WB protocol for FXYD1 antibody 67150-1-IgDownload protocol
IHC protocol for FXYD1 antibody 67150-1-IgDownload protocol
IF protocol for FXYD1 antibody 67150-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols