G3BP1 Monoclonal antibody

G3BP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E4A2

Cat no : 66486-1-Ig

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, pig brain tissue, RAW 264.7 cells, NIH/3T3 cells, PC-12 cells, HSC-T6 cells, K-562 cells, Jurkat cells, HepG2 cells, HEK-293 cells
Positive IHC detected inhuman testis tissue, human colon tissue, human lymphoma tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inEthacrynic acid treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66486-1-Ig targets G3BP1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen G3BP1 fusion protein Ag3728
Full Name GTPase activating protein (SH3 domain) binding protein 1
Calculated molecular weight 466 aa, 52 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC006997
Gene symbol G3BP1
Gene ID (NCBI) 10146
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocols

Product Specific Protocols
WB protocol for G3BP1 antibody 66486-1-IgDownload protocol
IHC protocol for G3BP1 antibody 66486-1-IgDownload protocol
IF protocol for G3BP1 antibody 66486-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanWB

J Exp Clin Cancer Res

Circ_0119872 promotes uveal melanoma development by regulating the miR-622/G3BP1 axis and downstream signalling pathways.

Authors - Shuting Liu
  • KD Validated
humanWB

Front Vet Sci

Porcine Circovirus Type 3 Cap Inhibits Type I Interferon Induction Through Interaction With G3BP1.

Authors - Pengfei Zhang
humanIF,FC

Clin Exp Immunol

Microvesicles in active lupus nephritis show TLR9-dependent co-expression of galectin-3 binding protein and double-stranded DNA.

Authors - Niclas Stefan Rasmussen
humanWB,IF

Front Microbiol

PMID: 32082291

Authors - Yingcheng Zheng
humanWB,IF

J Virol

Typical Stress Granule Proteins Interact with the 3' Untranslated Region of Enterovirus D68 To Inhibit Viral Replication.

Authors - Jinyan Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Haibo (Verified Customer) (10-26-2020)

This is a excellent antibody to visualize stress granules tested in HEK293 and human fibroblasts.

  • Applications: Immunofluorescence
  • Cell Tissue Type: HEK293 and human fibroblasts
FH

Manohar (Verified Customer) (09-23-2020)

1% milk is used

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293
FH

David (Verified Customer) (01-13-2020)

Good signal in control cells and immunopositive puncta seen in response to stress (e.g. sodium arsenite).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: SH-SY5Y
FH

Azita (Verified Customer) (10-04-2019)

Assembly of stress granula upon treatment with sodium arsenite for 40 min. (It works great)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/400
  • Cell Tissue Type: NSC-34
G3BP1 Antibody Immunofluorescence, validation (1/400 dilution) in NSC-34 (Cat no:66486-1-Ig)
FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HEK293T for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T
FH

Natalia (Verified Customer) (06-06-2019)

PFA fixated cells

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hepa1-6 cell line
G3BP1 Antibody Immunofluorescence, validation (1:1000 dilution) in Hepa1-6 cell line (Cat no:66486-1-Ig)