|Positive WB detected in||HeLa cells, mouse brain tissue, pig brain tissue, RAW 264.7 cells, NIH/3T3 cells, PC-12 cells, HSC-T6 cells, K-562 cells, Jurkat cells, HepG2 cells, HEK-293 cells|
|Positive IHC detected in||human testis tissue, human lymphoma tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Ethacrynic acid treated HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66486-1-Ig targets G3BP1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||G3BP1 fusion protein Ag3728|
|Full Name||GTPase activating protein (SH3 domain) binding protein 1|
|Calculated molecular weight||466 aa, 52 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC006997|
|Gene ID (NCBI)||10146|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
Nat Struct Mol Biol
TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.
J Exp Clin Cancer Res
Circ_0119872 promotes uveal melanoma development by regulating the miR-622/G3BP1 axis and downstream signalling pathways.
Front Vet Sci
Porcine Circovirus Type 3 Cap Inhibits Type I Interferon Induction Through Interaction With G3BP1.
Clin Exp Immunol
Microvesicles in active lupus nephritis show TLR9-dependent co-expression of galectin-3 binding protein and double-stranded DNA.
Typical Stress Granule Proteins Interact with the 3' Untranslated Region of Enterovirus D68 To Inhibit Viral Replication.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Haibo (Verified Customer) (10-26-2020)
This is a excellent antibody to visualize stress granules tested in HEK293 and human fibroblasts.
Manohar (Verified Customer) (09-23-2020)
1% milk is used
David (Verified Customer) (01-13-2020)
Good signal in control cells and immunopositive puncta seen in response to stress (e.g. sodium arsenite).
Azita (Verified Customer) (10-04-2019)
Assembly of stress granula upon treatment with sodium arsenite for 40 min. (It works great)
Kyosuke (Verified Customer) (06-12-2019)
It works well on HEK293T for Western blot.
Natalia (Verified Customer) (06-06-2019)
PFA fixated cells