Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse brain tissue, pig brain tissue, RAW 264.7 cells, NIH/3T3 cells, PC-12 cells, HSC-T6 cells, K-562 cells, Jurkat cells, HepG2 cells, HEK-293 cells|
|Positive IHC detected in||human testis tissue, human colon tissue, human lymphoma tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||sodium arsenite treated HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66486-1-Ig targets G3BP1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||G3BP1 fusion protein Ag3728|
|Full Name||GTPase activating protein (SH3 domain) binding protein 1|
|Calculated molecular weight||466 aa, 52 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC006997|
|Gene ID (NCBI)||10146|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
Nat Struct Mol Biol
TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.
J Exp Clin Cancer Res
Circ_0119872 promotes uveal melanoma development by regulating the miR-622/G3BP1 axis and downstream signalling pathways.
Front Vet Sci
Porcine Circovirus Type 3 Cap Inhibits Type I Interferon Induction Through Interaction With G3BP1.
Life Sci Alliance
Integrated stress response restricts macrophage necroptosis.
Arch Biochem Biophys
FAM98A promotes resistance to 5-fluorouracil in colorectal cancer by suppressing ferroptosis.
Clin Exp Immunol
Microvesicles in active lupus nephritis show TLR9-dependent co-expression of galectin-3 binding protein and double-stranded DNA.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Haibo (Verified Customer) (10-26-2020)
This is a excellent antibody to visualize stress granules tested in HEK293 and human fibroblasts.
Manohar (Verified Customer) (09-23-2020)
1% milk is used
David (Verified Customer) (01-13-2020)
Good signal in control cells and immunopositive puncta seen in response to stress (e.g. sodium arsenite).
Azita (Verified Customer) (10-04-2019)
Assembly of stress granula upon treatment with sodium arsenite for 40 min. (It works great)
Kyosuke (Verified Customer) (06-12-2019)
It works well on HEK293T for Western blot.
Natalia (Verified Customer) (06-06-2019)
PFA fixated cells