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G3BP1 Monoclonal antibody

G3BP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E4A2

Cat no : 66486-1-Ig

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII



Tested Applications

Positive WB detected inLNCaP cells, RAW 264.7 cells, pig brain tissue, HeLa cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman testis tissue, human colon tissue, human lymphoma tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected insodium arsenite treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66486-1-Ig targets G3BP1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen G3BP1 fusion protein Ag3728
Full Name GTPase activating protein (SH3 domain) binding protein 1
Calculated molecular weight 466 aa, 52 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC006997
Gene symbol G3BP1
Gene ID (NCBI) 10146
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocols

Product Specific Protocols
WB protocol for G3BP1 antibody 66486-1-IgDownload protocol
IHC protocol for G3BP1 antibody 66486-1-IgDownload protocol
IF protocol for G3BP1 antibody 66486-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nature

DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome.

Authors - Parimal Samir
mouseIF

Nature

Galactosaminogalactan activates the inflammasome to provide host protection.

Authors - Benoit Briard
humanWB,IF,IP

Autophagy

Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules

Authors - Cuiwei Yang
humanIF

Nat Chem Biol

K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle.

Authors - Yuanyuan Yu
humanWB

Nat Commun

Muscleblind acts as a modifier of FUS toxicity by modulating stress granule dynamics and SMN localization.

Authors - Ian Casci
mouseIF

Dev Cell

DDX3X Suppresses the Susceptibility of Hindbrain Lineages to Medulloblastoma.

Authors - Deanna M Patmore

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Haibo (Verified Customer) (10-26-2020)

This is a excellent antibody to visualize stress granules tested in HEK293 and human fibroblasts.

  • Applications: Immunofluorescence
  • Cell Tissue Type: HEK293 and human fibroblasts
FH

Manohar (Verified Customer) (09-23-2020)

1% milk is used

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293
FH

David (Verified Customer) (01-13-2020)

Good signal in control cells and immunopositive puncta seen in response to stress (e.g. sodium arsenite).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: SH-SY5Y
FH

Azita (Verified Customer) (10-04-2019)

Assembly of stress granula upon treatment with sodium arsenite for 40 min. (It works great)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/400
  • Cell Tissue Type: NSC-34
G3BP1 Antibody Immunofluorescence, validation (1/400 dilution) in NSC-34 (Cat no:66486-1-Ig)
FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HEK293T for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T
FH

Natalia (Verified Customer) (06-06-2019)

PFA fixated cells

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hepa1-6 cell line
G3BP1 Antibody Immunofluorescence, validation (1:1000 dilution) in Hepa1-6 cell line (Cat no:66486-1-Ig)