G3BP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

G3BP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

52

CloneNo.

1E4A2

Reviews (6)

Cat no : 66486-1-Ig

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

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Validation Data Gallery

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  WB analysis using 66486-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66486-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of pig brain using 66486-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66486-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66486-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat brain using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 66486-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 66486-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (66486-1-Ig, Clone: 1E4A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: LNCaP cells, RAW 264.7 cells, pig brain tissue, HeLa cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human testis tissue, human colon tissue, human lymphoma tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: sodium arsenite treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IF: See 41 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 4 publications below
• RIP: See 1 publications below

Product Information

66486-1-Ig targets G3BP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: G3BP1 fusion protein Ag3728
• Full Name: GTPase activating protein (SH3 domain) binding protein 1
• Calculated Molecular Weight: 466 aa, 52 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC006997
• Gene Symbol: G3BP1
• Gene ID (NCBI): 10146
• RRID: AB_2819031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Publications

Species	Application	Title
mouse	WB,IF	Nature; DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome.; Authors - Parimal Samir
mouse	IF	Nature; Galactosaminogalactan activates the inflammasome to provide host protection.; Authors - Benoit Briard
human	IF	Sci Transl Med; Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy; Authors - Takahiko Nishiyama
mouse	IF	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB,IF,IP	Autophagy; Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules; Authors - Cuiwei Yang
human	IF	Nat Chem Biol; K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle.; Authors - Yuanyuan Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Haibo (Verified Customer) (10-26-2020)

This is a excellent antibody to visualize stress granules tested in HEK293 and human fibroblasts.

• Applications: Immunofluorescence
• Cell Tissue Type: HEK293 and human fibroblasts

FH

Manohar (Verified Customer) (09-23-2020)

1% milk is used

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

FH

David (Verified Customer) (01-13-2020)

Good signal in control cells and immunopositive puncta seen in response to stress (e.g. sodium arsenite).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: SH-SY5Y

FH

Azita (Verified Customer) (10-04-2019)

Assembly of stress granula upon treatment with sodium arsenite for 40 min. (It works great)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: NSC-34

G3BP1 Antibody Immunofluorescence, validation (1/400 dilution) in NSC-34 (Cat no:66486-1-Ig)

FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HEK293T for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

FH

Natalia (Verified Customer) (06-06-2019)

PFA fixated cells

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hepa1-6 cell line

G3BP1 Antibody Immunofluorescence, validation (1:1000 dilution) in Hepa1-6 cell line (Cat no:66486-1-Ig)