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G3BP1 Monoclonal antibody
G3BP1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Human, mouse, rat, pig and More (1)
WB, IP, IHC, IF, FC, CoIP, ELISA
Cat no : 66486-1-Ig
Validation Data Gallery
|Positive WB detected in||LNCaP cells, RAW 264.7 cells, pig brain tissue, HeLa cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells|
|Positive IHC detected in||human testis tissue, human colon tissue, human lymphoma tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||sodium arsenite treated HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 12 publications below|
|IF||See 21 publications below|
|IP||See 2 publications below|
|FC||See 2 publications below|
|CoIP||See 1 publications below|
66486-1-Ig targets G3BP1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||G3BP1 fusion protein Ag3728|
|Full Name||GTPase activating protein (SH3 domain) binding protein 1|
|Calculated molecular weight||466 aa, 52 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC006997|
|Gene ID (NCBI)||10146|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
|Product Specific Protocols|
|WB protocol for G3BP1 antibody 66486-1-Ig||Download protocol|
|IHC protocol for G3BP1 antibody 66486-1-Ig||Download protocol|
|IF protocol for G3BP1 antibody 66486-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome.
Galactosaminogalactan activates the inflammasome to provide host protection.
Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules
Nat Chem Biol
K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle.
Muscleblind acts as a modifier of FUS toxicity by modulating stress granule dynamics and SMN localization.
DDX3X Suppresses the Susceptibility of Hindbrain Lineages to Medulloblastoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Haibo (Verified Customer) (10-26-2020)
This is a excellent antibody to visualize stress granules tested in HEK293 and human fibroblasts.
Manohar (Verified Customer) (09-23-2020)
1% milk is used
David (Verified Customer) (01-13-2020)
Good signal in control cells and immunopositive puncta seen in response to stress (e.g. sodium arsenite).
Azita (Verified Customer) (10-04-2019)
Assembly of stress granula upon treatment with sodium arsenite for 40 min. (It works great)
Kyosuke (Verified Customer) (06-12-2019)
It works well on HEK293T for Western blot.
Natalia (Verified Customer) (06-06-2019)
PFA fixated cells