CoraLite®488-conjugated G3BP1 Monoclonal antibody

G3BP1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1E4A2

Cat no : CL488-66486

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66486-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66486 targets G3BP1 in IF applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen G3BP1 fusion protein Ag3728
Full Name GTPase activating protein (SH3 domain) binding protein 1
Calculated molecular weight 466 aa, 52 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC006997
Gene symbol G3BP1
Gene ID (NCBI) 10146
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocols

Product Specific Protocols
IF protocol for CL488 G3BP1 antibody CL488-66486Download protocol
Standard Protocols
Click here to view our Standard Protocols