GABARAPL1 Monoclonal antibody

• Featured Product
• KD/KO Validated

GABARAPL1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1E10F6

Cat no : 66458-1-Ig

Synonyms

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GEC 1, GEC1

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Validation Data Gallery

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  WB analysis of mouse brain using 66458-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66458-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66458-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66458-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GABARAPL1 antibody (66458-1-Ig, Clone: 1E10F6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, pig brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

66458-1-Ig targets GABARAPL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GABARAPL1 fusion protein Ag1473
• Full Name: GABA(A) receptor-associated protein like 1
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC009309
• Gene Symbol: GABARAPL1
• Gene ID (NCBI): 23710
• RRID: AB_2881827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine.

Publications

Species	Application	Title
human	WB,IF	PLoS Pathog; The mitophagy receptor NIX induces vIRF-1 oligomerization and interaction with GABARAPL1 for the promotion of HHV-8 reactivation-induced mitophagy; Authors - Mai Tram Vo
human	WB	Iran J Basic Med Sci; Targeting GABARAPL1/HIF-2a axis to induce tumor cell apoptosis in nasopharyngeal carcinoma; Authors - Xiaopeng Huang ; KD Validated