GCNT1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 83121-4-RR (same clone as 83121-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83121-4-RR (GCNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83121-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83121-4-PBS

  Biolayer interferometry (BLl) kinetic assays of 83121-4-RR against Human GCNT1 were performed. The affinity constant is below 1 pM.

GCNT1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83121-4-PBS

Clone No.240071E9

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

Core 2 beta-1,6-N-acetylglucosaminyltransferase, C2GNT-L, C2GNT1, C2GlcNAcT, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

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Product Information

83121-4-PBS targets GCNT1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12264

  Product name: Recombinant human GCNT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 41-428 aa of BC074885

  Sequence: VSVRHLELAGENPSSDINCTKVLQGDVNEIQKVKLEILTVKFKKRPRWTPDDYINMTSDCSSFIKRRKYIVEPLSKEEAEFPIAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDTKSEDSYLAAVMGIASCFSNVFVASRLESVVYASWSRVQADLNCMKDLYAMSANWKYLINLCGMDFPIKTNLEIVRKLKLLMGENNLETERMPSHKEERWKKRYEVVNGKLTNTGTVKMLPPLETPLFSGSAYFVVSREYVGYVLQNEKIQKLMEWAQDTYSPDEYLWATIQRIPEVPGSLPASHKYDLSDMQAVARFVKWQYFEGDVSKGAPYPPCDGVHVRSVCIFGAGDLNWMLRKHHLFANKFDVDVDLFAIQCLDEHLRHKALETLKH

• Full Name: glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase)
• Calculated Molecular Weight: 428 aa, 50 kDa
• GenBank Accession Number: BC074885
• Gene Symbol: GCNT1
• Gene ID (NCBI): 2650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q02742
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Glucosaminyl (N-acetyl) transferase 1 (GCNT1, also known as C2GNT1), is a glycosyltransferase enzyme that plays an essential role in the formation of core 2 branched O-glycans that is crucial to the final definition of O-glycan structure (PMID: 37813880). GCNT1 promotes the growth of prostate tumors and regulates oncogenic gene expression pathways that are important for disease progression (PMID: 24854630).