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  • 0.5x10^6 GGGGS-transfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002) (red) or Rabbit IgG Isotype Control (98136-1-RR) (blue). 0.5x10^6 untransfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002-GMP) (black dashed). All cells were then stained with CL647-conjugated Donkey Anti-Rabbit Secondary (SA00014-7) at 1:500. Propidium Iodide was included in the final resuspension buffer at 1:1000.​ Cells were gated on Live (PI-), then the cell population in FSC vs. SSC, then single cells, then for positive signal in the APC-A channel.

    0.5x10^6 GGGGS-transfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002) (red) or Rabbit IgG Isotype Control (98136-1-RR) (blue). 0.5x10^6 untransfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002-GMP) (black dashed). All cells were then stained with CL647-conjugated Donkey Anti-Rabbit Secondary (SA00014-7) at 1:500. Propidium Iodide was included in the final resuspension buffer at 1:1000.​ Cells were gated on Live (PI-), then the cell population in FSC vs. SSC, then single cells, then for positive signal in the APC-A channel.

  • 0.5x10^6 GGGGS-transfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002) (red) or Rabbit IgG Isotype Control (98136-1-RR) (blue). 0.5x10^6 untransfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002-GMP) (black dashed). All cells were then stained with CL647-conjugated Donkey Anti-Rabbit Secondary (SA00014-7) at 1:500. Propidium Iodide was included in the final resuspension buffer at 1:1000.​ Cells were gated on Live (PI-), then the cell population in FSC vs. SSC, then single cells, then for positive signal in the APC-A channel.

    0.5x10^6 GGGGS-transfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002) (red) or Rabbit IgG Isotype Control (98136-1-RR) (blue). 0.5x10^6 untransfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002-GMP) (black dashed). All cells were then stained with CL647-conjugated Donkey Anti-Rabbit Secondary (SA00014-7) at 1:500. Propidium Iodide was included in the final resuspension buffer at 1:1000.​ Cells were gated on Live (PI-), then the cell population in FSC vs. SSC, then single cells, then for positive signal in the APC-A channel.

  • 0.5x10^6 GGGGS-transfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002) (red) or Rabbit IgG Isotype Control (98136-1-RR) (blue). 0.5x10^6 untransfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002-GMP) (black dashed). All cells were then stained with CL647-conjugated Donkey Anti-Rabbit Secondary (SA00014-7) at 1:500. Propidium Iodide was included in the final resuspension buffer at 1:1000.​ Cells were gated on Live (PI-), then the cell population in FSC vs. SSC, then single cells, then for positive signal in the APC-A channel.

    0.5x10^6 GGGGS-transfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002) (red) or Rabbit IgG Isotype Control (98136-1-RR) (blue). 0.5x10^6 untransfected HEK293 cells were stained with 0.5ug Anti-GGGGS Recombinant Monoclonal Antibody (CT-1002-GMP) (black dashed). All cells were then stained with CL647-conjugated Donkey Anti-Rabbit Secondary (SA00014-7) at 1:500. Propidium Iodide was included in the final resuspension buffer at 1:1000.​ Cells were gated on Live (PI-), then the cell population in FSC vs. SSC, then single cells, then for positive signal in the APC-A channel.

GGGGS Recombinant Monoclonal Antibody

Cat No. CT-1002

Review Product

Isotype

Rabbit IgG

Reactivity

human

Applications

Cell Activation

Product type:  Research use only
Size

-/ -

Freight/Packing: -

Quantity

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Product Information

Tested Reactivity human
IsoType Rabbit IgG
Class Recombinant
Immunogen KLH conjugated GGGGSGGGGSGGGGS
Conjugate Unconjugated
Format Liquid
Formulation 1XPBS
Storage Buffer 1XPBS
Storage Conditions Store at -20°C to -80°C
Shipping Condition Blue ice

Product Specifications

TestMethodSpecification
EndotoxinTested in accordance with USP <85><0.1 EU/μg
IdentitySDS-PAGE25kDa and 50kDa bands in reducing conditions
Mycoplasmatested in accordance with USP <63>Not Detected
PuritySDS-PAGE followed by denitometric analysis>95%
SpecificityT cell activation>5-fold over unactivated

Background

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S, G4S*2, G4S*3, G4S*4, and G4S*5.

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