Alpha galactosidase A Polyclonal antibody

• 

  WB analysis of HEK-293 using 19877-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19877-1-AP (Alpha galactosidase A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19877-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19877-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 19877-1-AP

  Immunofluorescent analysis of HepG2 cells, using GLA antibody 19877-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Alpha galactosidase A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 19877-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

GLA, Galactosylgalactosylglucosylceramidase GLA, GALA, EC:3.2.1.22, Alpha-galactosidase A

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19877-1-AP targets Alpha galactosidase A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7505

  Product name: Recombinant human GLA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 81-425 aa of BC002689

  Sequence: WKDAGYEYLCIDDCWMAPQRDSEGRLQADPQRFPHGIRQLANYVHSKGLKLGIYADVGNKTCAGFPGSFGYYDIDAQTFADWGVDLLKFDGCYCDSLENLADGYKHMSLALNRTGRSIVYSCEWPLYMWPFQKPNYTEIRQYCNHWRNFADIDDSWKSIKSILDWTSFNQERIVDVAGPGGWNDPDMLVIGNFGLSWNQQVTQMALWAIMAAPLFMSNDLRHISPQAKALLQDKDVIAINQDPLGKQGYQLRQGDNFEVWERPLSGLAWAVAMINRQEIGGPRSYTIAVASLGKGVACNPACFITQLLPVKRKLGFYEWTSRLRSHINPTGTVLLQLENTMQMSL

• Full Name: galactosidase, alpha
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC002689
• Gene Symbol: GLA
• Gene ID (NCBI): 2717
• RRID: AB_10642955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06280
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLA, also named as Melibiase, Agalsidas and Alpha-galactosidase A, Belongs to the glycosyl hydrolase 27 family. It hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Biol Sci; Septin4 promotes cell death in human colon cancer cells by interacting with BAX.; Authors - Xin Zhao