GLUT2 Monoclonal antibody

GLUT2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1G8B1

Cat no : 66889-1-Ig

Synonyms

GLUT 2, GLUT2, SLC2A2

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Validation Data Gallery

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  WB analysis of 4T1 using 66889-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66889-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66889-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66889-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 66889-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66889-1-Ig (GLUT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66889-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66889-1-Ig (GLUT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: 4T1 cells, HSC-T6 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66889-1-Ig targets GLUT2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GLUT2 fusion protein Ag14551
• Full Name: solute carrier family 2 (facilitated glucose transporter), member 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC060041
• Gene Symbol: SLC2A2
• Gene ID (NCBI): 6514
• RRID: AB_2918478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLUT2 is a member of glucose transporters or GLUTs which catalyze the glucose transport across plasma membrane. GLUT2 is selectively expressed in hepatocytes, pancreatic beta cells, and absorptive renal and intestinal epithelial cells. GLUT2 is required to maintain normal glucose homeostasis and normal function and development of the endocrine pancreas. Its absence leads to symptoms characteristic of non-insulin-dependent diabetes mellitus.

Publications

Species	Application	Title
mouse	IF	Sci Rep; Regulatory mechanisms of glucose absorption in the mouse proximal small intestine during fasting and feeding; Authors - Chisato Nakamura
human	IHC	Oncol Lett; Prediction and verification of the prognostic biomarker SLC2A2 and its association with immune infiltration in gastric cancer; Authors - Weijian Zhang