Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cellls, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67777-1-Ig targets GOLPH3 in WB, IHC, IF/ICC, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5443 Product name: Recombinant human GOLPH3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-298 aa of BC033725 Sequence: MTSLTQRSSGLVQRRTEASRNAADKERAAGGGAGSSEDDAQSRRDEQDDDDKGDSKETRLTLMEEVLLLGLKDREGYTSFWNDCISSGLRGCMLIELALRGRLQLEACGMRRKSLLTRKVICKSDAPTGDVLLDEALKHVKETQPPETVQNWIELLSGETWNPLKLHYQLRNVRERLAKNLVEKGVLTTEKQNFLLFDMTTHPLTNNNIKQRLIKKVQEAVLDKWVNDPHRMDRRLLALIYLAHASDVLENAFAPLLDEQYDLATKRVRQLLDLDPEVECLKANTNEVLWAVVAAFTK Predict reactive species |
| Full Name | golgi phosphoprotein 3 (coat-protein) |
| Calculated Molecular Weight | 298 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC033725 |
| Gene Symbol | GOLPH3 |
| Gene ID (NCBI) | 64083 |
| RRID | AB_2918542 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H4A6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signaling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GOLPH3 antibody 67777-1-Ig | Download protocol |
| IHC protocol for GOLPH3 antibody 67777-1-Ig | Download protocol |
| WB protocol for GOLPH3 antibody 67777-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GOLPH3 Monoclonal antibody (3H2A5), catalog number 67777-1-Ig, has 4 citations published in J Adv Res, Advanced Science, Cell Death & Disease, and Science Advances. The cited research spans inflammation and acute pancreatitis (macrophage PANoptosis), osteoarthritis and lipid metabolism reprogramming, non-small-cell lung cancer metastasis via exosomal WNT3A secretion, and Golgi maturation cargo selection mechanisms.
| Species | Application | Title |
|---|---|---|
J Adv Res Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion | ||
Adv Sci (Weinh) Sequential Targeting Chondroitin Sulfate-Bilirubin Nanomedicine Attenuates Osteoarthritis via Reprogramming Lipid Metabolism in M1 Macrophages | ||
Cell Death Dis GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer | ||
Reviews
What customers say
The single available review for this product reports a negative experience. The user tested the antibody for immunofluorescence and observed no specific staining at all, resulting in a 1-star rating. No additional details regarding dilution, tissue type, or troubleshooting steps were provided in the review.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (12-06-2022) | No specific staining at all.
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