GOLPH3 Monoclonal antibody

GOLPH3 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3H2A5

Cat no : 67777-1-Ig

Synonyms

Coat protein GPP34, FLJ90675, Golgi phosphoprotein 3, GOLPH3, GOPP1, GPP34, MIDAS



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cellls, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67777-1-Ig targets GOLPH3 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GOLPH3 fusion protein Ag5443
Full Name golgi phosphoprotein 3 (coat-protein)
Calculated molecular weight 298 aa, 34 kDa
GenBank accession numberBC033725
Gene symbol GOLPH3
Gene ID (NCBI) 64083
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signalling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

Protocols

Product Specific Protocols
WB protocol for GOLPH3 antibody 67777-1-IgDownload protocol
IHC protocol for GOLPH3 antibody 67777-1-IgDownload protocol
IF protocol for GOLPH3 antibody 67777-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols