CoraLite®594-conjugated GOLPH3 Monoclonal antibody

GOLPH3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H2A5

Cat no : CL594-67777

Synonyms

Coat protein GPP34, FLJ90675, Golgi phosphoprotein 3, GOLPH3, GOPP1, GPP34, MIDAS



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67777-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67777 targets GOLPH3 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GOLPH3 fusion protein Ag5443
Full Name golgi phosphoprotein 3 (coat-protein)
Calculated molecular weight 298 aa, 34 kDa
GenBank accession numberBC033725
Gene symbol GOLPH3
Gene ID (NCBI) 64083
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signalling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

Protocols

Product Specific Protocols
IF protocol for CL594 GOLPH3 antibody CL594-67777Download protocol
Standard Protocols
Click here to view our Standard Protocols