GPD2 Monoclonal antibody

GPD2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C12E2

Cat no : 68174-1-Ig

Synonyms

GDH2, GPD M, GPD2, GPDH M, GPDM, mGPDH, mtGPD

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Validation Data Gallery

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  WB analysis using 68174-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 68174-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68174-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68174-1-Ig targets GPD2 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPD2 fusion protein Ag11212
• Full Name: glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• Calculated Molecular Weight: 41 kDa, 81 kDa
• GenBank Accession Number: BC019874
• Gene Symbol: GPD2
• Gene ID (NCBI): 2820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).