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GPT2 Polyclonal antibody
GPT2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 16757-1-AP
Validation Data Gallery
|Positive WB detected in
|mouse liver tissue, mouse kidney tissue, HepG2 cells
|Positive IP detected in
|mouse kidney tissue
|Positive IHC detected in
|human skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:3000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16757-1-AP targets GPT2 in WB, IP, IHC, IF, FC (Intra), FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|GPT2 fusion protein Ag10273
|glutamic pyruvate transaminase (alanine aminotransferase) 2
|Calculated molecular weight
|523 aa, 58 kDa
|Observed molecular weight
|58 kDa, 47 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.
|Product Specific Protocols
|WB protocol for GPT2 antibody 16757-1-AP
|IHC protocol for GPT2 antibody 16757-1-AP
|IF protocol for GPT2 antibody 16757-1-AP
|IP protocol for GPT2 antibody 16757-1-AP
|Click here to view our Standard Protocols
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.
Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.
Proc Natl Acad Sci U S A
Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.
Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2