GPT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

33

Cat no : 16757-1-AP

Synonyms

ALAT2, Alanine aminotransferase 2, Glutamic--pyruvic transaminase 2, Glutamic--alanine transaminase 2, EC:2.6.1.2

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Validation Data Gallery

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  WB analysis using 16757-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16757-1-AP (GPT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16757-1-AP

  WB result of GPT2 antibody (16757-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPT2 transfected HepG2 cells.

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  WB analysis of mouse kidney using 16757-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16757-1-AP (GPT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16757-1-AP

  IP result of anti-GPT2 (IP:16757-1-AP, 4ug; Detection:16757-1-AP 1:500) with mouse kidney tissue lysate 7200ug.

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  IHC staining of human skeletal muscle using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16757-1-AP (GPT2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16757-1-AP (GPT2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16757-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT2 antibody (16757-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 16757-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GPT2 (16757-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse kidney tissue, HepG2 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human skeletal muscle tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• ELISA: See 1 publications below

Product Information

16757-1-AP targets GPT2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPT2 fusion protein Ag10273
• Full Name: glutamic pyruvate transaminase (alanine aminotransferase) 2
• Calculated Molecular Weight: 523 aa, 58 kDa
• Observed Molecular Weight: 58 kDa, 47 kDa
• GenBank Accession Number: BC062555
• Gene Symbol: GPT2
• Gene ID (NCBI): 84706
• RRID: AB_2112098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.

Publications

Species	Application	Title
		Cancer Cell; Inosine induces stemness features in CAR-T cells and enhances potency; Authors - Dorota D Klysz
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
mouse	WB	Cell Res; Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.; Authors - Wei-Feng Cai ; KD Validated
human	WB	Nat Commun; Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.; Authors - Yun-Qian Wang
human	WB	Cancer Res; Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.; Authors - Bo Zhang
MOUSE	WB,IF	Proc Natl Acad Sci U S A; Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.; Authors - Qing Ouyang

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