CoraLite® Plus 647 Anti-Human Glycophorin A/CD235a Rabbit Recombinant Antibody

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  FC experiment of Human peripheral blood erythrocytes using CL647-98370-2

  1x10^6 Human peripheral blood erythrocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human Glycophorin A/CD235a Rabbit RecAb (CL647-98370-2, Clone: 242822E1) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

Glycophorin A/CD235a Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98370-2

Clone No.242822E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Glycophorin A, CD235a, Glycophorin-A, GPA, GPErik

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Tested Applications

• Positive FC detected in: Human peripheral blood erythrocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98370-2 targets Glycophorin A/CD235a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Glycophorin A/CD235a fusion protein Eg2336
• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: Glycophorin A
• Gene ID (NCBI): 2993
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02724
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)