Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-66006 targets HA Tag in WB applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | HA Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3086576 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | HATAG |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Publications
What published studies show
HRP-66006 is the HRP-conjugated HA Tag Monoclonal antibody (clone 1F5C6). It has 5 citations appearing in Cell, Cell Death & Disease, New Phytologist, Communications Biology, and Journal of Cancer. The associated research fields include STING signaling and COPII vesicle trafficking, tumor progression in esophageal squamous cell carcinoma, cell survival signaling in Chlamydomonas, ciliary trafficking regulation, and lung adenocarcinoma progression.
| Species | Application | Title |
|---|---|---|
Cell Death Dis GPR37-enhanced ubiquitination of ATP1A1 inhibits tumor progression and radiation resistance in esophageal squamous cell carcinoma | ||
New Phytol A RAF-SnRK2-PP2C functional module regulates cell survival in the chlorophyte Chlamydomonas reinhardtii. | ||
Commun Biol Rab23 GTPase and IFT43 regulate the trafficking of prostaglandin E receptor 4 to primary cilia. | ||
J Cancer KRT80 Promotes Lung Adenocarcinoma Progression and Serves as a Substrate for VCP |
