Tested Applications
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66006 targets HA Tag in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | HA Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2935607 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | HATAG |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 HA Tag antibody CL594-66006 | Download protocol |
| IF protocol for CL594 HA Tag antibody CL594-66006 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL594-66006 is the HA Tag Monoclonal antibody (1F5C6) conjugated with CoraLite® Plus 594. It has a total of 2 citations. One appears in Nature Communications (IF 18.1), studying cardiac homeostasis and mitochondrial oxidative respiration via immunofluorescence. The other is in Bioresource Technology (IF 8.2), related to yeast surface display system construction. The research fields span cardiology/mitochondrial biology and biotechnology/fermentation engineering.
| Species | Application | Title |
|---|---|---|
Nat Commun The transcriptional repressor HEY2 regulates mitochondrial oxidative respiration to maintain cardiac homeostasis | ||
Bioresour Technol CEN-Display: Construction and optimization of a surface display system in Saccharomyces cerevisiae CEN.PK2-1C. |
