HA Tag Monoclonal antibody

HA Tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (7)

Applications

WB, IF/ICC, IP, CoIP, ChIP, ELISA, PLA

Conjugate

Unconjugated

CloneNo.

1F5C6

Cat no : 66006-2-Ig

Synonyms

HA Tag, YPYDVPDYA


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  • Western Blot (WB) analysis of HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of HEK-293 using 66006-2-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.1μg/mL.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.05μg/mL.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.025μg/mL.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.0125μg/mL.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    IP experiment of Transfected HEK-293 using 66006-2-Ig

    IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    IF Staining of Transfected HEK-293 using 66006-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

"HA Tag Antibodies" Comparison

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Tested Applications

Positive WB detected inTransfected HEK-293 cells, HEK-293 cells, Ag19172
Positive IP detected inTransfected HEK-293 cells
Positive IF/ICC detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66006-2-Ig targets HA Tag in WB, IF/ICC, IP, CoIP, ChIP, ELISA, PLA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, rat, pig, canine, yeast, duck
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name HA Tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Protocols

Product Specific Protocols
WB protocol for HA Tag antibody 66006-2-IgDownload protocol
IF protocol for HA Tag antibody 66006-2-IgDownload protocol
IP protocol for HA Tag antibody 66006-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell

SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9

Authors - Nora Schmidt

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
humanIP

Nat Commun

Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis

Authors - Zhikai Wang
IP

J Clin Invest

FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway

Authors - Yunjiang Zhou
WB

Nat Microbiol

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Authors - Zhimin Jiang
WB

Acta Pharm Sin B

Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.

Authors - Xiliang Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Parijat (Verified Customer) (03-06-2024)

Good antibody for immunofluorescence. Low background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: 293T Cells