"HA Tag Antibodies" Comparison
View side-by-side comparison of HA Tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Transfected HEK-293 cells, HEK-293 cells, Ag19172 |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66006-2-Ig targets HA Tag in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, PLA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat, recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | HA Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881490 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| IF protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| IP protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| WB protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HA Tag Monoclonal antibody (Cat# 66006-2-Ig) has garnered 539 citations across prestigious journals including Nature, Science, Cell, Cell Metabolism, Nature Communications, Autophagy, Cell Death & Differentiation, Molecular Cell, Advanced Science, and Oncogene. Associated research fields encompass oncology, autophagy, ferroptosis, immunology, neurodegeneration, virology, metabolism, cardiology, liver disease, and plant biology, demonstrating its wide applicability in protein detection and functional validation studies.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma | ||
J Hematol Oncol METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma | ||
Science DNA-protein cross-links promote cGAS-STING-driven premature aging and embryonic lethality. |
Reviews
What customers say
Most users report positive results with this HA tag antibody. Two reviewers gave it 5 stars, noting it works very well for Western Blot at 1:5,000 dilution and performs well in immunofluorescence at 1:250 with low background in 293T cells. One reviewer gave 1 star, reporting non-specific labeling in Western Blot at 1:10,000 dilution in HEK cells. Overall, the antibody is generally well-received for detecting HA-tagged proteins, though one user experienced specificity issues at a higher dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (07-21-2026) | Non-specific labelling
|
Sai Sindhura (Verified Customer) (01-08-2026) | HA tag works very well
|
Parijat (Verified Customer) (03-06-2024) | Good antibody for immunofluorescence. Low background.
|
