HA Tag Monoclonal antibody

HA Tag Monoclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (4)

Applications

WB, PLA, IP, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

1F5C6

Cat no : 66006-2-Ig

Synonyms

HA Tag, YPYDVPDYA


"HA Tag Antibodies" Comparison

View side-by-side comparison of HA Tag antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • Western Blot (WB) analysis of HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of HEK-293 using 66006-2-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    IP experiment of Transfected HEK-293 using 66006-2-Ig

    IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    IF Staining of Transfected HEK-293 using 66006-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    FC experiment of Transfected HEK-293 using 66006-2-Ig

    1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti- HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

  • Flow cytometry (FC) experiment of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    FC experiment of Transfected HEK-293 using 66006-2-Ig

    1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti-N/A HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

  • Flow cytometry (FC) experiment of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    FC experiment of Transfected HEK-293 using 66006-2-Ig

    1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti-N/A HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.


Tested Applications

Positive WB detected inTransfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells
Positive FC detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66006-2-Ig targets HA Tag in WB, PLA, IP, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name HA Tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Protocols

Product Specific Protocols
WB protocol for HA Tag antibody 66006-2-IgDownload protocol
IF protocol for HA Tag antibody 66006-2-IgDownload protocol
IP protocol for HA Tag antibody 66006-2-IgDownload protocol
FC protocol for HA Tag antibody 66006-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
humanIP

Nat Commun

Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis

Authors - Zhikai Wang
WB

Nat Microbiol

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Authors - Zhimin Jiang
mouseWB

Sci Adv

Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage

Authors - Dayun Feng
humanCoIP,ChIP

Nat Commun

CDK9 activity switch associated with AFF1 and HEXIM1 controls differentiation initiation from epidermal progenitors

Authors - Sarah M. Lloyd
WB,IP

Cell Death Differ

Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma

Authors - Yu-Fei Cao