HA Tag Monoclonal antibody
HA Tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (7)
Applications
WB, IF/ICC, IP, CoIP, ChIP, ELISA, PLA
Conjugate
Unconjugated
219
CloneNo.
1F5C6
Cat no : 66006-2-Ig
Synonyms
"HA Tag Antibodies" Comparison
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Tested Applications
Positive WB detected in | Transfected HEK-293 cells, HEK-293 cells, Ag19172 |
Positive IP detected in | Transfected HEK-293 cells |
Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:10000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 154 publications below |
IF | See 23 publications below |
IP | See 51 publications below |
ELISA | See 1 publications below |
CoIP | See 24 publications below |
ChIP | See 2 publications below |
Product Information
66006-2-Ig targets HA Tag in WB, IF/ICC, IP, CoIP, ChIP, ELISA, PLA applications and shows reactivity with recombinant protein samples.
Tested Reactivity | recombinant protein |
Cited Reactivity | human, mouse, rat, pig, canine, yeast, duck |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | HA Tag |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2881490 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HA Tag antibody 66006-2-Ig | Download protocol |
IF protocol for HA Tag antibody 66006-2-Ig | Download protocol |
IP protocol for HA Tag antibody 66006-2-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9 | ||
Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. | ||
Nat Commun Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis | ||
J Clin Invest FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway | ||
Nat Microbiol IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. | ||
Acta Pharm Sin B Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Parijat (Verified Customer) (03-06-2024) | Good antibody for immunofluorescence. Low background.
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