HNRNPAB Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 84207-1-RR (same clone as 84207-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84207-1-RR (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

  WB result of HNRNPAB antibody (84207-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPAB transfected HeLa cells. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 84207-1-RR (same clone as 84207-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01139-1

  Cytometric bead array standard curve of MP01139-1, HNRNPAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84207-1-PBS. Detection antibody: 84207-2-PBS. Standard: Ag35010. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01139-2

  Cytometric bead array standard curve of MP01139-2, HNRNPAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84207-1-PBS. Detection antibody: 84207-3-PBS. Standard: Ag35010. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84207-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84207-1-PBS against Human HNRNPAB was performed. The affinity constant is 2.01 nM.

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  Affinity and Kinetic Characterization of 84207-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84207-1-RR against Human HNRNPAB were performed. The affinity constant is 1.07 nM.

• Featured Product
• KD/KO Validated

HNRNPAB Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 84207-1-PBS

Clone No.241462B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

241462B1, ABBP 1, ABBP1, ABBP-1, APOBEC1-binding protein 1

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Product Information

84207-1-PBS targets HNRNPAB as part of a matched antibody pair:

MP01139-1: 84207-1-PBS capture and 84207-2-PBS detection (validated in Cytometric bead array)

MP01139-2: 84207-1-PBS capture and 84207-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag35010

  Product name: Recombinant human HNRNPAB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-62 aa of BC036708

  Sequence: MSEAGEEQPMETTGATENGHEAVPEGESPAGAGTGAAAGAGGATAAPPSGNQNGAEGDQINA

• Full Name: heterogeneous nuclear ribonucleoprotein A/B
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36-42 kDa
• GenBank Accession Number: BC036708
• Gene Symbol: HNRNPAB
• Gene ID (NCBI): 3182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99729
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).