ChromoTek Histone-Label Atto488

Validation Data Gallery

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  Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

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  Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

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  Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

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  ChromoTek Histone-Label is a convenient, ready to use, and high-performing chromatin staining probe with low background levels and that differentiates between euchromatin and heterochromatin. HeLa cells stained in parallel with Histone-Label (1.25 mg/L) and a monoclonal rabbit anti-H2A IgG / anti-rabbit Alexa568 secondary antibody. Histone-Label co-localizes with conventional antibody staining, however Histone-Label better penetrates into the compactly packed nuclei than the anti-H2A IgG and secondary IgGs, which are one order of magnitude larger than the Histone-Label: see more green signal from Histone-Label at center of nuclei and more red signal from anti-H2A IgG on surface/edge of nuclei of merge image on very right.

Histone-Label* is a fluorescent chromatin probe for direct staining of chromosomes and nuclei. It consists of an anti-histone VHH conjugated to the fluorescent dye ATTO488. For IF, microscopy of histones, chromosomes, and nuclei.

Cat No. tba488

Publications(5)

Specificity

Histone H2A-H2B heterodimers

Applications

IF, ICC

Conjugate

ATTO 488

Host

Alpaca

Synonyms

Histone-Label, Histone, Histone H2A, Histone H2B, Histone H2A-H2B

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Product Information

Histone-Label* is a fluorescent chromatin probe for direct staining of chromosomes and nuclei. It consists of an anti-histone VHH conjugated to the fluorescent dye ATTO488. For IF, microscopy of histones, chromosomes, and nuclei.

• Description: Histone-Label* immunostains chromatin throughout the cell cycle (interphase chromatin, chromatin condensation and mitotic chromosomes) due to specific binding to histone H2A-H2B heterodimers:

  • Specific probe for direct immunostaining of histones, chromosomes, and nuclei

  • Labels histones throughout the nuclei

  • Better penetration into densely packed nuclei

  • Higher image resolution

  • Monovalent VHHs do not cluster their epitopes

  *Commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment
• Applications: IF, ICC
• Host: Alpaca
• Specificity/Target: Histone-Label immunostains chromatin throughout the cell cycle (interphase chromatin, chromatin condensation and mitotic chromosomes) due to specific binding to histone H2A-H2B heterodimers.
• Conjugate: ATTO 488
• Physical State: Liquid
• Suggested Dilution: IF/ICC: 1:400
• Type: Nanobody
• Class: Recombinant
• RRID: AB_2827588
• Excitation/ Emission: Excitation range 480 - 510 nm (λ_abs= 501 nm) ; Emission range 520 - 560 nm (λ_fl= 523 nm)
• Storage Buffer: 1x PBS, 0.09% sodium azide.
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light.
• Size: 10 μL; 100 μL
• Note: This product is for research use only, not for diagnostic or therapeutic use

Commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment

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Publications

Application	Title
IF	Adv Mater; Hydrogel-Induced Cell Membrane Disruptions Enable Direct Cytosolic Delivery of Membrane-Impermeable Cargo.; Authors - Jelter Van Hoeck
IHC	Nat Biomed Eng; Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies.; Authors - Nobuyuki Tanaka
IF	Biophys J; DNA length tunes the fluidity of DNA-based condensates.; Authors - Fernando Muzzopappa
IF	Nano Res; Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation.; Authors - Jing Liu
	JCI Insight; Three-dimensional imaging of upper tract urothelial carcinoma improves diagnostic yield and accuracy; Authors - Keishiro Fukumoto