Speedy™ Human APOE One-Step ELISA Kit
Cat no : SE50006
Synonyms
Apo E, Apo-E, apoe4, apolipoprotein E, ApolipoproteinE
Validation Data Gallery
Product Information
SE50006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOE ELISA kit is to be used to detect and quantify protein levels of endogenous APOE. The assay recognizes human APOE. An antibody specific for APOE has been pre-coated onto the microwells. Add samples and standards to the well. At the same time, another horseradish peroxidase (HRP)-conjugated antibody specific for APOE is added to detect the captured APOE protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Speedy™ Human APOE One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma,Cell culture supernatant, Urine, Human Milk |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL, 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 348 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 103% | 82%-126% |
| Cell culture supernatants | 105% | 81%-126% |
| Urine | 95% | 80%-113% |
| Human milk | 104% | 89%-121% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 9.66 | 0.18 | 1.88 |
| 2 | 20 | 1.73 | 0.04 | 2.38 |
| 3 | 20 | 0.41 | 0.02 | 3.97 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.38 | 0.19 | 2.08 |
| 2 | 24 | 1.76 | 0.04 | 2.15 |
| 3 | 24 | 0.46 | 0.01 | 2.15 |
Background Information
APOE (apolipoprotein E) is a plasma protein implicated in lipid metabolism. It is produced predominantly by hepatocytes, macrophages, and neural cells. In the CNS, APOE is the major extracellular lipid carrier and plays a key role in neuronal protection and repair after injury. APOE is polymorphic, with three major alleles: APOE2 (cys112, cys158), APOE3 (cys112, arg158), and APOE4 (arg112, arg158). APOE2 plays a protective role against both Alzheimer's (AD) and heart disease. APOE4 confers a higher risk for atherosclerosis and Alzheimer's disease. Analysis of cerebrospinal fluid (CSF) and plasma APOE protein levels in AD patients had been performed to examine its association with AD pathology. In old age, high plasma APOE levels precede an increase of circulating CRP and strongly associates with cardiovascular mortality.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Apo E, Apo-E, apoe4, apolipoprotein E, ApolipoproteinE |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
Publications
| Species | Sample Type | Title |
|---|---|---|
ACS Biomater Sci Eng Liposomal-Cannabidiol Nanoformulation to Suppress HIV Replication and Reduce Oxidative Stress in Infected Microglia. | ||
J Mol Neurosci Multilayer Validation Reveals a Glia-Associated Secretome Signature in Temporal Lobe Epilepsy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Adriana (Verified Customer) (12-13-2024) | It is easy and rapid to perform. The supernatants diluted 1:50, 1:100, and 1:150 recovered substantial APOE protein for analysis. The blanks were clear and demonstrated no unspecific reaction. My only concern is the RPM proposed for incubation. I could not reach 500 rpm, so I used a maximum of 250 rpm.
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