Recombinant Human CCL15 protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2877

---

Validation Data Gallery

• 

  Purity of Recombinant Human CCL15 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human CCL15 was greater than 90% as determined by SEC-HPLC.

---

Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CCL15 protein Ser46-Ile113 (Accession# Q16663) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 6359
• Accession: Q16663
• PredictedSize: 33.5 kDa
• SDS-PAGE: 34-37 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CCL15, also known as macrophage inhibitory protein 5 (MIP-5), leukocyte chemokine 1 (Lkn-1), and human CC chemokine 2 (HCC-2), is a small cytokine that belongs to the CC chemokine family. This family of chemokines is located on human chromosome 17 and shares a gene sequence similarity with CC motif chemokine ligand 5 (CCL5) and CC motif chemokine ligand 3 (CCL3). CCL15 is expressed in certain leukocytes and macrophages in the liver, small intestine, colon, and lung. Chemokines are a class of small molecule proteins that bind to the corresponding cell surface receptors and participate in a variety of physiological, pathological, and immunological processes (PMID: 30881115).

References:

1. Zu G. et al. (2019). Cancer Manag Res. 11:1881-1892.