Anti-Human Ig Lambda Light Chain Rabbit Recombinant Antibody

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  IHC staining of human tonsillitis using 98723-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98723-2-RR (Human Ig, λ Light Chain antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of human PBMCs using 98723-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Ig Lambda Light Chain Rabbit RecAb (98723-2-RR, Clone: 251497D8) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 750 Anti-Human CD19. The cells originate from CD11b-negative cells. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of human PBMCs using 98723-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Ig Lambda Light Chain Rabbit RecAb (98723-2-RR, Clone: 251497D8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). The cells originate from CD11b-negative cells. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Ig Lambda Light Chain Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98723-2-RR

Clone No.251497D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

Ig Lambda, Ig λ Light Chain, IGHG1, Immunoglobulin heavy constant gamma 1, Lambda Light Chain

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: human PBMCs

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98723-2-RR targets Ig Lambda Light Chain in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6676

  Product name: recombinant human IgG1-IgL1 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10009502 of

  Sequence: /

• Full Name: immunoglobulin heavy constant gamma 1 (G1m marker)
• Gene Symbol: IGHG1
• Gene ID (NCBI): 3500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Human Ig lambda (λ) light chain is one of the two types of light chains (the other being kappa, κ) that constitute the basic building blocks of human antibodies (immunoglobulins). Each antibody molecule contains two identical light chains paired with two identical heavy chains, forming the characteristic Y-shaped structure. It is an essential component of the humoral immune system, providing structural diversity for antigen recognition while serving as a critical diagnostic marker for plasma cell dyscrasias.