Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-98723-2 targets Ig Lambda Light Chain in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6676 Product name: recombinant human IgG1-IgL1 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Sequence: / Predict reactive species |
| Full Name | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Gene Symbol | IGHG1 |
| Gene ID (NCBI) | 3500 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Human Ig lambda (λ) light chain is one of the two types of light chains (the other being kappa, κ) that constitute the basic building blocks of human antibodies (immunoglobulins). Each antibody molecule contains two identical light chains paired with two identical heavy chains, forming the characteristic Y-shaped structure. It is an essential component of the humoral immune system, providing structural diversity for antigen recognition while serving as a critical diagnostic marker for plasma cell dyscrasias.
