Human IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

1A1A2

Cat no : 66408-1-Ig

Synonyms

Human IgG4, IgG4, IGHG4



Tested Applications

Positive WB detected inhuman plasma tissue, human heart tissue, human skeletal muscle tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66408-1-Ig targets Human IgG4 in WB, IHC, IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberAL928742
Gene symbol IGHG4
Gene ID (NCBI) 3503
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.

Protocols

Product Specific Protocols
WB protocol for Human IgG4 antibody 66408-1-IgDownload protocol
IHC protocol for Human IgG4 antibody 66408-1-IgDownload protocol
IF protocol for Human IgG4 antibody 66408-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols