Human IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1A1A2

Cat no : 66408-1-Ig

Synonyms

Human IgG4, IgG4, IGHG4

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Validation Data Gallery

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  WB analysis of human plasma using 66408-1-Ig

  human plasma (0.4 nL) were subjected to SDS PAGE followed by western blot with 66408-1-Ig (human IgG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66408-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66408-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66408-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66408-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human appendicitis using 66408-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG4 antibody (66408-1-Ig, Clone: 1A1A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma tissue, human heart tissue, human skeletal muscle tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human appendicitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66408-1-Ig targets Human IgG4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: immunoglobulin heavy constant gamma 4 (G4m marker)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: AL928742
• Gene Symbol: IGHG4
• Gene ID (NCBI): 3503
• RRID: AB_2881780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.

Publications

Species	Application	Title
	IF	Breast Cancer Res; Clinical and biological heterogeneities in triple-negative breast cancer reveals a non-negligible role of HER2-low; Authors - Xi E Hu