Biotin-conjugated IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Conjugate

Biotin

CloneNo.

1A1A2

Cat no : Biotin-66408

Synonyms

IgG4, IGHG4

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Validation Data Gallery

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  IHC staining of human tonsillitis using Biotin-66408

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66408 (IGHG4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-66408

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66408 (IGHG4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66408 targets Human IgG4 in IHC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: immunoglobulin heavy constant gamma 4 (G4m marker)
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: AL928742
• Gene Symbol: IGHG4
• Gene ID (NCBI): 3503
• RRID: AB_2883071
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4. This antibody is Biotin conjugated.