Biotin-conjugated Human IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Conjugate

Biotin Fluorescent Dye

CloneNo.

1A1A2

Cat no : Biotin-66408

Synonyms

IgG4, IGHG4



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

Biotin-66408 targets Human IgG4 in IHC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Calculated molecular weight 55 kDa
GenBank accession numberAL928742
Gene symbol IGHG4
Gene ID (NCBI) 3503
Conjugate Biotin Fluorescent Dye
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4. This antibody is Biotin conjugated.

Protocols

Product Specific Protocols
IHC protocol for Biotin Human IgG4 antibody Biotin-66408Download protocol
Standard Protocols
Click here to view our Standard Protocols