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CoraLite® Plus 647-conjugated IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1A1A2

Cat no : CL647-66408

Synonyms

Human IgG4, IGHG4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 647-conjugated Human IgG4 Monoclona (CL647-66408)

    IF Staining of human tonsillitis using CL647-66408

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 Human IgG4 antibody (CL647-66408, Clone: 1A1A2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 647-conjugated Human IgG4 Monoclona (CL647-66408)

    IF Staining of human tonsillitis using CL647-66408

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 Human IgG4 antibody (CL647-66408, Clone: 1A1A2 ) at dilution of 1:100.

Tested Applications

Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66408 targets Human IgG4 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberAL928742
Gene Symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2920282
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Human IgG4 antibody CL647-66408Download protocol
Standard Protocols
Click here to view our Standard Protocols