Tested Applications
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66408 targets Human IgG4 in IF-P applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | immunoglobulin heavy constant gamma 4 (G4m marker) |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | AL928742 |
| Gene Symbol | IGHG4 |
| Gene ID (NCBI) | 3503 |
| RRID | AB_2883328 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Human IgG4 antibody CL488-66408 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CoraLite® Plus 488-conjugated Human IgG4 Monoclonal antibody (CL488-66408) has 2 total citations. They appear in Cell and STAR Protoc. The research fields involve autoimmune kidney disease—specifically autoantibody-triggered podocyte membrane budding—and a protocol for isolating human extracellular vesicles from nephrotic urine to enrich autoimmunoglobulin-triggered extracellular vesicles. Both studies focus on human autoimmunity and nephrotic/extracellular vesicle biology.
| Species | Application | Title |
|---|---|---|
STAR Protoc Protocol for isolating human extracellular vesicles from nephrotic urine and enriching autoimmunoglobulin-triggered extracellular vesicles. |
