CD122/IL-2RB Monoclonal antibody

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  WB analysis using 60174-1-Ig

  Untreated and PNGase F-treated lysates of NK-92 cells were subjected to SDS PAGE followed by western blot with 60174-1-Ig (CD122/IL2RB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 60174-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60174-1-Ig (IL2RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CD122/IL-2RB Monoclonal Antibody for WB, ELISA

Cat No. 60174-1-Ig

Clone No.3A12C7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

IL2RB, 3A12C7, CD122, High affinity IL-2 receptor subunit beta, IL 2 receptor subunit beta

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Tested Applications

• Positive WB detected in: NK-92 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60174-1-Ig targets CD122/IL-2RB in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3929

  Product name: Recombinant human IL-2RB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 270-551 aa of BC025691

  Sequence: TGPWLKKVLKCNTPDPSKFFSQLSSEHGGDVQKWLSSPFPSSSFSPGGLAPEISPLEVLERDKVTQLLLQQDKVPEPASLSSNHSLTSCFTNQGYFFFHLPDALEIEACQVYFTYDPYSEEDPDEGVAGAPTGSSPQPLQPLSGEDDAYCTFPSRDDLLLFSPSLLGGPSPPSTAPGGSGAGEERMPPSLQERVPRDWDPQPLGPPTPGVPDLVDFQPPPELVLREAGEEVPDAGPREGVSFPWSRPPGQGEFRALNARLPLNTDAYLSLQELQGQDPTHLV

• Full Name: interleukin 2 receptor, beta
• Calculated Molecular Weight: 551 aa, 61 kDa
• Observed Molecular Weight: 75 kDa, 61 kDa
• GenBank Accession Number: BC025691
• Gene Symbol: IL2RB
• Gene ID (NCBI): 3560
• ENSEMBL Gene ID: ENSG00000100385
• RRID: AB_3085029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P14784
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.