IL-5 Polyclonal antibody

• 

  WB analysis of Recombinant protein using 17786-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17786-1-AP (IL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IL5 Polyclonal Antibody for WB, ELISA

Cat No. 17786-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EDF, IL 5, IL5, IL-5, Interleukin 5, T cell replacing factor, TRF

---

Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17786-1-AP targets IL5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11840

  Product name: Recombinant human IL-5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 24-134 aa of BC066282

  Sequence: IPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

• Full Name: interleukin 5 (colony-stimulating factor, eosinophil)
• Calculated Molecular Weight: 134 aa, 15 kDa
• GenBank Accession Number: BC066282
• Gene Symbol: IL5
• Gene ID (NCBI): 3567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05113
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-5, or IL-5, was originally discovered as a soluble T cell-derived factor, called T cell-replacing factor (TRF), that induced T cell-depleted activated B cells to secrete immunoglobulin. IL-5 is a key hematopoietic cytokine in eosinophil differentiation, maturation, recruitment and activation at sites of allergic inflammation. IL-5 also plays a role in the development, metabolism, and function of basophils. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of at least two chains: an α chain that binds IL-5 with low affinity and a β chain that does not bind IL-5, but together with the IL-5α chain, constitutes the high affinity IL-5 receptor. The β chain is common to the IL-3, IL-5 and GM-CSF receptors and has been shown to signal through the JAK/Stat pathway. IL-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed.