IRAK3 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

  WB result of IRAK3 antibody (83876-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK3 transfected THP-1 cells. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 83876-2-RR (same clone as 83876-2-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 83876-2-RR (same clone as 83876-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRAK3 antibody (83876-2-RR, Clone: 240975C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83876-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83876-2-PBS against Human IRAK3 was performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 83876-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83876-2-RR against Human IRAK3 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

IRAK3 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 83876-2-PBS

Clone No.240975C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

240975C5, ASRT5, IL-1 receptor-associated kinase M, Inactive IL-1 receptor-associated kinase 3, Interleukin-1 receptor-associated kinase 3

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Product Information

83876-2-PBS targets IRAK3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4959

  Product name: Recombinant human IRAK3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 251-596 aa of BC057800

  Sequence: DRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSVICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLWYMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRELMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNLLPSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVPSQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDEYEQYKKE

• Full Name: interleukin-1 receptor-associated kinase 3
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC057800
• Gene Symbol: IRAK3
• Gene ID (NCBI): 11213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y616
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IRAK3 (Interleukin-1 receptor-associated kinase-3, also named IRAKM) as a kinase-deficient member of the TLR/IRAK family has been shown to negatively regulate Toll-like receptor (TLR)-mediated cell signaling (PMID: 33590831).