IFN-beta Polyclonal antibody

IFN-beta Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 27506-1-AP

Synonyms

IFN beta, IFNB1, Interferon beta, IFN-β1, IFNB

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Validation Data Gallery

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  WB analysis of Recombinant protein using 27506-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 27506-1-AP (IFN Beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using IFN Beta antibody (27506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27506-1-AP (IFN-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IF/ICC detected in: L02 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

27506-1-AP targets IFN-beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFN-beta fusion protein Ag26326
• Full Name: IFN beta
• Calculated Molecular Weight: 187 aa, 22 kDa
• GenBank Accession Number: BC069314
• Gene Symbol: IFN beta1
• Gene ID (NCBI): 3456
• RRID: AB_2880893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon-beta (IFN-β) is a type I interferon, which is a group of signaling proteins made and released by host cells in response to the presence of several viruses, including double-stranded RNA produced by many viruses. IFN-β is a single protein species and is molecularly related to IFN-α subtypes, although it is antigenically distinct from them. It is mainly produced by fibroblasts and plays a crucial role in the innate immune response against viruses. IFN-β can inhibit viral replication by interfering with the virus's RNA or DNA, thus suppressing viral growth. It also enhances the cytotoxic activity of natural killer (NK) cells and promotes the expression of major histocompatibility complex (MHC) class I molecules.

Publications

Species	Application	Title
human	IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
human	WB	Int Immunopharmacol; Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways.; Authors - Juan-Juan Li
human	WB	Amino Acids; Escherichia coli infection activates the production of IFN-α and IFN-β via the JAK1/STAT1/2 signaling pathway in lung cells.; Authors - Yan Jin
human	WB	BMC Vet Res; The role of IBV PL1pro in virus replication and suppression of host innate immune responses; Authors - Weirong Liu
human	IF	Cell Death Dis; Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion.; Authors - Tongtian Zhuang
		Virology; Role of heparan sulfate in the Zika virus entry, replication, and cell death.; Authors - Huixin Gao