Validation Data Gallery
|Positive IHC detected in||human tonsillitis tissue, K-562 cells, Insulinoma tissue, human lung cancer tissue, human breast cancer tissue, human skin cancer tissue, human lymphoma tissue, human colon cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HEK-293 cells|
|Positive FC detected in||Jurkat cells|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:10000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 27309-1-AP is KI67 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, goat, rabbit, hamster, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KI67 fusion protein Ag26266|
|Full Name||antigen identified by monoclonal antibody Ki-67|
|Calculated molecular weight||359 kDa|
|GenBank accession number||NM_002417|
|Gene ID (NCBI)||4288|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.
Signal Transduct Target Ther
FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth
lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2
Stress Granules determine the Development of Obesity-associated Pancreatic Cancer.
Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.
Enzyme-Triggered Transcytosis of Dendrimer-Drug Conjugate for Deep Penetration into Pancreatic Tumors.
C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guo-rong (Verified Customer) (08-30-2022)
Alessandro (Verified Customer) (07-27-2022)
No aspecific staining, great outcome
Ana (Verified Customer) (02-15-2022)
Both, 1:50 and 1:100 dilutions worked well
kes (Verified Customer) (01-17-2022)
Works at 1:200 dilution for IHC
Yan (Verified Customer) (02-18-2020)
1:100 work for cell.