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- KD/KO Validated
KI67 Polyclonal antibody
KI67 Polyclonal Antibody for FC, IF, IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IHC, IF, FC, ELISA
Conjugate
Unconjugated
320
Cat no : 27309-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human gliomas tissue, human lung cancer tissue, human lymphoma tissue, human skin cancer tissue, Insulinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2000-1:10000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 42 publications below |
| IHC | See 246 publications below |
| IF | See 52 publications below |
Product Information
The immunogen of 27309-1-AP is KI67 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | hamster, human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KI67 fusion protein Ag26266 |
| Full Name | antigen identified by monoclonal antibody Ki-67 |
| Calculated molecular weight | 359 kDa |
| GenBank accession number | NM_002417 |
| Gene symbol | MKI67 |
| Gene ID (NCBI) | 4288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KI67 antibody 27309-1-AP | Download protocol |
| IF protocol for KI67 antibody 27309-1-AP | Download protocol |
| FC protocol for KI67 antibody 27309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dose Response Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin. | ||
Nat Commun The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer. | ||
Cell Death Dis NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6. | ||
In Vitro Cell Dev Biol Anim miR-145-5p suppresses osteogenic differentiation of adipose-derived stem cells by targeting semaphorin 3A. | ||
Cancer Med The preoperative neutrophil-to-lymphocyte ratio is not a marker of prostate cancer characteristics but is an independent predictor of biochemical recurrence in patients receiving radical prostatectomy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Yan (Verified Customer) (02-18-2020) | 1:100 work for cell.
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