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KI67 Antibody 59 Publications

Rabbit Polyclonal| Catalog number: 27309-1-AP

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Con: 90 μg/150 μl

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Tested Applications

Applications:
IHC, IF, ELISA
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Images See all

KI67 Antibody IHC human tonsillitis tissue 27309-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 27309-1-AP( KI67 antibody) at dilution of 1:1000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

KI67 Antibody IHC human tonsillitis tissue 27309-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 27309-1-AP( KI67 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

KI67 Antibody IF HeLa cells 27309-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 27309-1-AP(KI67 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human
Positive IHC detected in:
human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human gliomas tissue, human lung cancer tissue, human lymphoma tissue, human skin cancer tissue, Insulinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
IHC : 1:4000-1:10000
IF : 1:50-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
KI67 fusion protein Ag26266
Full name:
antigen identified by monoclonal antibody Ki-67
Calculated molecular weight:
359 kDa
Observed molecular weight:
GenBank accession number:
NM_002417
Gene ID (NCBI):
4288
Gene symbol
MKI67
Synonyms
Antigen KI 67, Ki 67, KI67, KIA, MKI67

Background

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.


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