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  • KD/KO Validated

KI67 Polyclonal antibody

KI67 Polyclonal Antibody for FC, IF, IHC,ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 27309-1-AP


Antigen KI 67, Ki 67, KI67, KIA, MKI67

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human gliomas tissue, human lung cancer tissue, human lymphoma tissue, human skin cancer tissue, Insulinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27309-1-AP is KI67 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity hamster, human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KI67 fusion protein Ag26266
Full Name antigen identified by monoclonal antibody Ki-67
Calculated molecular weight 359 kDa
GenBank accession numberNM_002417
Gene symbol MKI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.


Product Specific Protocols
IHC protocol for KI67 antibody 27309-1-APDownload protocol
IF protocol for KI67 antibody 27309-1-APDownload protocol
FC protocol for KI67 antibody 27309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Dose Response

Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.

Authors - Xin Jiang

Front Pharmacol

PMID: 30670971

Authors - Zhang-Qi Cao

Nat Commun

The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.

Authors - Weimei Tang

Cell Death Dis

NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6.

Authors - Chunjie Xu

In Vitro Cell Dev Biol Anim

miR-145-5p suppresses osteogenic differentiation of adipose-derived stem cells by targeting semaphorin 3A.

Authors - Xiangdong Liu

Cancer Med

The preoperative neutrophil-to-lymphocyte ratio is not a marker of prostate cancer characteristics but is an independent predictor of biochemical recurrence in patients receiving radical prostatectomy.

Authors - Zhi Cao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Yan (Verified Customer) (02-18-2020)

1:100 work for cell.

  • Applications: Immunofluorescence,