|Positive IHC detected in||human tonsillitis tissue, Insulinoma tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, human gliomas tissue, human skin cancer tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Immunohistochemistry (IHC)||IHC : 1:8000-1:20000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 27309-1-AP is KI67 Fusion Protein expressed in E. coli.
|Cited Reactivity||hamster, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KI67 fusion protein Ag26266|
|Full Name||antigen identified by monoclonal antibody Ki-67|
|Calculated molecular weight||359 kDa|
|GenBank accession number||NM_002417|
|Gene ID (NCBI)||4288|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yan (Verified Customer) (02-18-2020)
1:100 work for cell.