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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated KI67 Polyclonal antibody

KI67 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-27309

Synonyms

Antigen KI 67, Ki 67, KI67, KIA, MKI67

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated KI67 Polyclonal antibody (CL555-27309)

    IF Staining of HeLa using CL555-27309

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 KI67 antibody (CL555-27309) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated KI67 Polyclonal antibody (CL555-27309)

    IF Staining of HeLa using CL555-27309

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 KI67 antibody (CL555-27309) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®555-conjugated KI67 Polyclonal antibody (CL555-27309)

    FC experiment of Jurkat using CL555-27309

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human KI67 (CL555-27309) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL555-27309 is KI67 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KI67 fusion protein Ag26266
Full Name antigen identified by monoclonal antibody Ki-67
Calculated Molecular Weight 359 kDa
GenBank Accession NumberNM_002417
Gene Symbol MKI67
Gene ID (NCBI) 4288
RRIDAB_2919649
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Protocols

Product Specific Protocols
IF protocol for CL555 KI67 antibody CL555-27309Download protocol
Standard Protocols
Click here to view our Standard Protocols